Detailed information of TA system TA144485

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3399544..3400146
• Replicon: chromosome
• Accession: NZ_CP046539
• Organism: Escherichia coli strain FHI_NMBU_10

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: GOE21_RS16760
• Protein ID: WP_000897305.1
• Coordinates: 3399835..3400146 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GOE21_RS16755
• Protein ID: WP_000356397.1
• Coordinates: 3399544..3399834 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GOE21_RS16730	3395489..3396391	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
GOE21_RS16735	3396388..3397023	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
GOE21_RS16740	3397020..3397949	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
GOE21_RS16745	3398279..3398521	-	243	WP_001087409.1	hypothetical protein	-
GOE21_RS16750	3398740..3398958	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
GOE21_RS16755	3399544..3399834	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
GOE21_RS16760	3399835..3400146	-	312	WP_000897305.1	hypothetical protein	Toxin
GOE21_RS16765	3400375..3401283	+	909	WP_001162704.1	alpha/beta hydrolase	-
GOE21_RS16770	3401347..3402288	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
GOE21_RS16775	3402333..3402770	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
GOE21_RS16780	3402767..3403639	-	873	WP_000920762.1	virulence factor BrkB family protein	-
GOE21_RS16785	3403633..3404232	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T144485 WP_000897305.1 NZ_CP046539:c3400146-3399835 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T144485 NZ_CP061975:c4569037-4568504 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT144485 WP_000356397.1 NZ_CP046539:c3399834-3399544 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT144485 NZ_CP061975:c4569314-4569048 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7