Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2271798..2272717 Replicon chromosome
Accession NZ_CP046529
Organism Mycobacterium tuberculosis strain SIT745/EAI1-MYS

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag AYJ03_RS10650 Protein ID WP_003900449.1
Coordinates 2272112..2272717 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag AYJ03_RS10645 Protein ID WP_003410124.1
Coordinates 2271798..2272103 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AYJ03_RS10615 2266809..2268065 - 1257 WP_003410095.1 HNH endonuclease -
AYJ03_RS10620 2268419..2268994 + 576 WP_003410100.1 hypothetical protein -
AYJ03_RS10625 2268991..2270031 + 1041 WP_003911735.1 ImmA/IrrE family metallo-endopeptidase -
AYJ03_RS10630 2270273..2270992 + 720 WP_003410108.1 DUF433 domain-containing protein -
AYJ03_RS10635 2270982..2271398 + 417 WP_003410114.1 hypothetical protein -
AYJ03_RS10640 2271414..2271713 - 300 WP_003410120.1 hypothetical protein -
AYJ03_RS10645 2271798..2272103 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
AYJ03_RS10650 2272112..2272717 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AYJ03_RS10655 2272742..2273101 - 360 WP_003410131.1 hypothetical protein -
AYJ03_RS10660 2273261..2273938 - 678 WP_003900450.1 hypothetical protein -
AYJ03_RS10665 2273890..2274126 - 237 WP_003410133.1 hypothetical protein -
AYJ03_RS10670 2274333..2275229 - 897 WP_003410136.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2264358..2275229 10871


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T144438 WP_003900449.1 NZ_CP046529:c2272717-2272112 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T144438 NZ_CP061961:746769-747254 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT144438 WP_003410124.1 NZ_CP046529:c2272103-2271798 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT144438 NZ_CP061961:746479-746772 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References