Detailed information of TA system TA144390

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 4672489..4672714
• Replicon: chromosome
• Accession: NZ_CP046527
• Organism: Escherichia coli strain RM19259

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: GN312_RS23270
• Protein ID: WP_000813258.1
• Coordinates: 4672489..4672644 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	4672656..4672714 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GN312_RS23220	4667492..4667923	-	432	WP_001302123.1	tellurite resistance protein	-
GN312_RS23235	4668374..4669087	-	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
GN312_RS23240	4669223..4669420	-	198	WP_000917763.1	hypothetical protein	-
GN312_RS23245	4669645..4670199	-	555	WP_000640035.1	DUF1133 family protein	-
GN312_RS23250	4670262..4670567	-	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
GN312_RS23255	4670580..4671629	-	1050	WP_001265229.1	DUF968 domain-containing protein	-
GN312_RS23260	4671631..4671903	-	273	WP_000191872.1	hypothetical protein	-
GN312_RS23265	4672025..4672369	-	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
GN312_RS23270	4672489..4672644	-	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	4672656..4672714	+	59	-	-	Antitoxin
GN312_RS23275	4672935..4673492	-	558	WP_000104474.1	DUF551 domain-containing protein	-
GN312_RS23280	4673494..4673712	-	219	WP_000683609.1	DUF4014 family protein	-
GN312_RS23285	4673840..4674151	-	312	WP_001289673.1	hypothetical protein	-
GN312_RS23290	4674144..4674371	-	228	WP_000699809.1	hypothetical protein	-
GN312_RS23295	4674368..4674649	-	282	WP_000603384.1	DNA-binding protein	-
GN312_RS23300	4674682..4675398	-	717	WP_000450627.1	DUF1627 domain-containing protein	-
GN312_RS23305	4675432..4675893	-	462	WP_000139447.1	replication protein	-
GN312_RS23310	4675886..4676929	-	1044	WP_001262402.1	hypothetical protein	-
GN312_RS23315	4676998..4677423	-	426	WP_000693878.1	Rha family transcriptional regulator	-
GN312_RS23320	4677407..4677649	-	243	WP_000747948.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	4642034..4741103	99069

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T144390 WP_000813258.1 NZ_CP046527:c4672644-4672489 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T144390 NZ_CP061943:c4115794-4115513 [Pseudomonas chlororaphis]
ATGACTTATGAGCTGGAGTTCTCCGAGAAAGCCTGGAAGGAATGGCAAAAGCTGGGCCCCACGCTGAAAGAGCAATTCAA
GAAGAAGCTGCAGGAACGACTCGTCAACCCCCATGTCCCGGCCGATCGCCTGAGCGGTTTGGGCAATGCCTACAAAATCA
AACTGCGCACGGCGGGCTACCGCCTGGTGTATCGGGTGGTGGATGAGGTGTTGGTGGTGACGGTCATTGCCGTGGGCAAG
CGAGAGCGGGGGAGTGTTTATCGGCAAGCCAGCAAGCGTTAG

Antitoxin

Nucleotide

• Length: 59 bp

>AT144390 NZ_CP046527:4672656-4672714 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin