Detailed information of TA system TA144380

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 2908118..2908812
• Replicon: chromosome
• Accession: NZ_CP046527
• Organism: Escherichia coli strain RM19259

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: GN312_RS14495
• Protein ID: WP_001263495.1
• Coordinates: 2908414..2908812 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: GN312_RS14490
• Protein ID: WP_000554757.1
• Coordinates: 2908118..2908411 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GN312_RS14470	2903829..2904326	+	498	Protein_2708	REP-associated tyrosine transposase RayT	-
GN312_RS14475	2904471..2906183	-	1713	Protein_2709	flagellar biosynthesis protein FlhA	-
GN312_RS14480	2906155..2906940	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
GN312_RS14485	2907011..2908066	+	1056	WP_001226186.1	DNA polymerase IV	-
GN312_RS14490	2908118..2908411	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
GN312_RS14495	2908414..2908812	+	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
GN312_RS14500	2908822..2909274	+	453	WP_001059871.1	GNAT family N-acetyltransferase	-
GN312_RS14505	2909592..2909795	+	204	Protein_2715	RNA ligase RtcB family protein	-
GN312_RS14510	2909794..2910315	+	522	Protein_2716	peptide chain release factor H	-
GN312_RS14515	2910372..2911829	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
GN312_RS14520	2912090..2912548	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	2913144..2913224	+	81	NuclAT_11	-	-
-	2913144..2913224	+	81	NuclAT_11	-	-
-	2913144..2913224	+	81	NuclAT_11	-	-
-	2913144..2913224	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T144380 WP_001263495.1 NZ_CP046527:2908414-2908812 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T144380 NZ_CP046527:2908414-2908812 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT144380 WP_000554757.1 NZ_CP046527:2908118-2908411 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT144380 NZ_CP046527:2908118-2908411 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6