Detailed information of TA system TA144374

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-agrB/SymE(toxin)
• Location: 2554519..2554931
• Replicon: chromosome
• Accession: NZ_CP046527
• Organism: Escherichia coli strain RM19259

Toxin (Protein)

• Gene name: symE
• Uniprot ID: Q8FA88
• Locus tag: GN312_RS12890
• Protein ID: WP_000132630.1
• Coordinates: 2554519..2554860 (-)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	agrB
Locus tag	-
Coordinates	2554855..2554931 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GN312_RS28010	2550087..2550287	+	201	WP_001310455.1	hypothetical protein	-
GN312_RS12870	2550530..2551942	-	1413	WP_000199295.1	PLP-dependent aminotransferase family protein	-
GN312_RS12875	2552119..2552283	+	165	WP_000394274.1	DUF1127 domain-containing protein	-
GN312_RS12880	2552380..2553261	+	882	WP_001304007.1	DUF262 domain-containing protein	-
GN312_RS12885	2553309..2554472	+	1164	WP_001304006.1	DUF1524 domain-containing protein	-
GN312_RS12890	2554519..2554860	-	342	WP_000132630.1	endoribonuclease SymE	Toxin
-	2554855..2554931	+	77	NuclAT_14	-	Antitoxin
-	2554855..2554931	+	77	NuclAT_14	-	Antitoxin
-	2554855..2554931	+	77	NuclAT_14	-	Antitoxin
-	2554855..2554931	+	77	NuclAT_14	-	Antitoxin
-	2554855..2554931	+	77	NuclAT_15	-	Antitoxin
-	2554855..2554931	+	77	NuclAT_15	-	Antitoxin
-	2554855..2554931	+	77	NuclAT_15	-	Antitoxin
-	2554855..2554931	+	77	NuclAT_15	-	Antitoxin
GN312_RS12895	2555081..2556835	-	1755	WP_000110082.1	restriction endonuclease subunit S	-
GN312_RS12900	2556835..2558303	-	1469	Protein_2400	N-6 DNA methylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002655 Escherichia coli O157:H7 str. EDL933 5456776..5475497 0 View NC_002695 Escherichia coli O157:H7 str. Sakai 5426911..5445631 0	inside	Genomic island	-	-	2544012..2562731	18719

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12294.13 Da
• Isoelectric Point: 8.4982

>T144374 WP_000132630.1 NZ_CP046527:c2554860-2554519 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T144374 NZ_CP061930:c3749096-3748878 [Klebsiella michiganensis]
ATGTCTGATAAAACATTAACCAAAATAGACTATTTGATGCGTTTACGACGCTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAAAATAAATATGAACTTTCCGATAATGAACTGGCGGTATTTTACTCTGCGGCCGATCATCGTCTTGCAGAGC
TGACGATGAATAAACTGTATGACAAGATCCCCCCTTCAGTCTGGAAATTTATCCGCTAG

Antitoxin

Nucleotide

• Length: 77 bp

>AT144374 NZ_CP046527:2554855-2554931 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A0E0Y5T0

Antitoxin