Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 1161644..1162443 Replicon chromosome
Accession NZ_CP046527
Organism Escherichia coli strain RM19259

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag GN312_RS06320 Protein ID WP_000347273.1
Coordinates 1161979..1162443 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID C3ST72
Locus tag GN312_RS06315 Protein ID WP_001302819.1
Coordinates 1161644..1161979 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GN312_RS06300 1157429..1158199 - 771 WP_001058231.1 2-dehydro-3-deoxyglucarate aldolase -
GN312_RS06305 1158215..1159549 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
GN312_RS06310 1159924..1161495 + 1572 WP_001273776.1 galactarate dehydratase -
GN312_RS06315 1161644..1161979 + 336 WP_001302819.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
GN312_RS06320 1161979..1162443 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
GN312_RS06325 1162498..1163307 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
GN312_RS06330 1163556..1164836 + 1281 WP_000681927.1 tagatose-bisphosphate aldolase subunit KbaZ -
GN312_RS06335 1164859..1165332 + 474 WP_001301475.1 PTS N-acetylgalactosamine transporter subunit IIB -
GN312_RS06340 1165343..1166122 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
GN312_RS06345 1166112..1166990 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
GN312_RS06350 1167008..1167442 + 435 WP_000948831.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T144366 WP_000347273.1 NZ_CP046527:1161979-1162443 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T144366 NZ_CP061915:67034-67183 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGTTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12388.97 Da        Isoelectric Point: 4.8616

>AT144366 WP_001302819.1 NZ_CP046527:1161644-1161979 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT144366 NZ_CP061915:c66988-66930 [Escherichia coli]
AGGAACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure

References