Detailed information of TA system TA144335

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4759921..4760510
• Replicon: chromosome
• Accession: NZ_CP046488
• Organism: Xanthomonas oryzae pv. oryzae strain IXO35

Toxin (Protein)

• Gene name: graT
• Uniprot ID: G7TIR7
• Locus tag: IXO35_RS22340
• Protein ID: WP_012443754.1
• Coordinates: 4760229..4760510 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IXO35_RS22335
• Protein ID: WP_011260746.1
• Coordinates: 4759921..4760211 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IXO35_RS22310 (IXO35_022385)	4755119..4755445	-	327	WP_237384726.1	hypothetical protein	-
IXO35_RS22315 (IXO35_022390)	4755514..4756548	-	1035	WP_011407587.1	IS630 family transposase	-
IXO35_RS22320 (IXO35_022395)	4756816..4757148	+	333	WP_011260743.1	hypothetical protein	-
IXO35_RS22325	4757308..4757495	+	188	Protein_4264	hypothetical protein	-
IXO35_RS22330 (IXO35_022405)	4757598..4758992	-	1395	WP_075240796.1	type III secretion system effector XopQ	-
IXO35_RS22335 (IXO35_022410)	4759921..4760211	-	291	WP_011260746.1	HigA family addiction module antitoxin	Antitoxin
IXO35_RS22340 (IXO35_022415)	4760229..4760510	-	282	WP_012443754.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IXO35_RS22345 (IXO35_022420)	4760605..4762863	-	2259	WP_075240794.1	exodeoxyribonuclease V subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11066.83 Da
• Isoelectric Point: 10.5318

>T144335 WP_012443754.1 NZ_CP046488:c4760510-4760229 [Xanthomonas oryzae pv. oryzae]
VIRSFIDKDAEKIWLGERSRRLPADIQLVARRKLRMLNAAAHLDDLRIPPANRLEALKGQRRGQYSIRINDQWRICFRWM
EGDVVQVEIVDYH

Nucleotide

• Length: 282 bp

>T144335 NZ_CP061868:208035-208385 [Priestia koreensis]
TTGGTAGTCAAACGTGGTGACGTCTATTTTGCTGATTTGTCTCCTGTAGTCGGCTCTGAACAAGGAGGCGTACGCCCAGT
ACTTATCATTCAAAATGACATTGGTAATCGGTTTAGCCCTACAGTAATTGTTGCTGCAATTACTGCTCAGATCCAGAAGG
CGAAACTTCCTACACATGTGGAAATTGATGCGAAACGGTACGGTTTTGAAAGAGACTCAGTGATTTTATTAGAACAAATT
CGTACAATTGACAAACAGCGCTTGACCGATAAAATTACTCATTTAGATGAGGAAATGATGGATCGCGTTGATAAGGCGTT
GCAAGTCAGTTTGGGACTTATTGATTTTTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10420.07 Da
• Isoelectric Point: 7.2040

>AT144335 WP_011260746.1 NZ_CP046488:c4760211-4759921 [Xanthomonas oryzae pv. oryzae]
MTVLPNIHPGDILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERFWLGLQADYELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT144335 NZ_CP061868:207748-208029 [Priestia koreensis]
GTGTCTGAAGTTAGTGCAACTACAGAAATTTTAGTTCGTTTACCTCAAAGCTTAGTATCTGAATTAGATGTGCTTGTAAA
GCAAGAGAATGGTAACCGAAGTGATTTTATTTATCAAGCAACGAAAATGTACCTTCGTGAGAGAAAGAAGCGTCATATCC
GTGAAACCATGAAGCGTGGATACATGGAAATGGCAAAAATTAATTTAAATATTGCATGTGAAGCTTTCCTAGCTGAGTAC
GAAGCAGATCATACTGTAGAACGCTTAGTTAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: A0A0K0GFW5