Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1137691..1138418 Replicon chromosome
Accession NZ_CP046446
Organism Leclercia sp. J807

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag GNG26_RS05275 Protein ID WP_156237979.1
Coordinates 1138107..1138418 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag GNG26_RS05270 Protein ID WP_156237978.1
Coordinates 1137691..1138110 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GNG26_RS05255 1132999..1133241 - 243 WP_103824118.1 DUF1158 domain-containing protein -
GNG26_RS05260 1133725..1136436 + 2712 WP_156237977.1 cation-transporting P-type ATPase -
GNG26_RS05265 1136514..1137635 - 1122 WP_103824120.1 glutamate--cysteine ligase -
GNG26_RS05270 1137691..1138110 - 420 WP_156237978.1 helix-turn-helix domain-containing protein Antitoxin
GNG26_RS05275 1138107..1138418 - 312 WP_156237979.1 type II toxin-antitoxin system HigB family toxin Toxin
GNG26_RS05280 1138742..1138987 + 246 WP_103178569.1 YmjA family protein -
GNG26_RS05285 1139083..1139571 + 489 WP_156237980.1 cold shock domain-containing protein -
GNG26_RS05290 1139799..1140512 + 714 WP_156237981.1 GNAT family N-acetyltransferase -
GNG26_RS22475 1141457..1141597 + 141 WP_197080127.1 hypothetical protein -
GNG26_RS05295 1141950..1142102 + 153 WP_156237982.1 type I toxin-antitoxin system Hok family toxin -
GNG26_RS05300 1142403..1143374 + 972 WP_156237983.1 type I-F CRISPR-associated endonuclease Cas1 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-136)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12481.49 Da        Isoelectric Point: 10.7380

>T144300 WP_156237979.1 NZ_CP046446:c1138418-1138107 [Leclercia sp. J807]
MHVVSRAPFEMATRRFPNQALALDDLYRVLKKENYTSPNQMRKRFPSLDRMKYREKWWVIDVGGGHLRVMFFADFARGRI
FIKHITTHAEYDKLTDIYRRTKE

Download         Length: 312 bp

>T144300 NZ_CP061842:3825972-3826190 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15442.58 Da        Isoelectric Point: 4.7657

>AT144300 WP_156237978.1 NZ_CP046446:c1138110-1137691 [Leclercia sp. J807]
MKYAEAINAANNLTNVLPFLGARPSRKDYEDALALVEYLLEHQPDSPLIDMLTAKIDKYESESPEFAEFNARIAGTPSGV
ALLRTLMDQYDLTQSDLENEIGKKSLVSRILNGQRTLTLDHMRALAKRFGLPVSAFVGD

Download         Length: 420 bp

>AT144300 NZ_CP061842:3825571-3825945 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References