Detailed information of TA system TA144300

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1137691..1138418
• Replicon: chromosome
• Accession: NZ_CP046446
• Organism: Leclercia sp. J807

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GNG26_RS05275
• Protein ID: WP_156237979.1
• Coordinates: 1138107..1138418 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GNG26_RS05270
• Protein ID: WP_156237978.1
• Coordinates: 1137691..1138110 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GNG26_RS05255	1132999..1133241	-	243	WP_103824118.1	DUF1158 domain-containing protein	-
GNG26_RS05260	1133725..1136436	+	2712	WP_156237977.1	cation-transporting P-type ATPase	-
GNG26_RS05265	1136514..1137635	-	1122	WP_103824120.1	glutamate--cysteine ligase	-
GNG26_RS05270	1137691..1138110	-	420	WP_156237978.1	helix-turn-helix domain-containing protein	Antitoxin
GNG26_RS05275	1138107..1138418	-	312	WP_156237979.1	type II toxin-antitoxin system HigB family toxin	Toxin
GNG26_RS05280	1138742..1138987	+	246	WP_103178569.1	YmjA family protein	-
GNG26_RS05285	1139083..1139571	+	489	WP_156237980.1	cold shock domain-containing protein	-
GNG26_RS05290	1139799..1140512	+	714	WP_156237981.1	GNAT family N-acetyltransferase	-
GNG26_RS22475	1141457..1141597	+	141	WP_197080127.1	hypothetical protein	-
GNG26_RS05295	1141950..1142102	+	153	WP_156237982.1	type I toxin-antitoxin system Hok family toxin	-
GNG26_RS05300	1142403..1143374	+	972	WP_156237983.1	type I-F CRISPR-associated endonuclease Cas1	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12481.49 Da
• Isoelectric Point: 10.7380

>T144300 WP_156237979.1 NZ_CP046446:c1138418-1138107 [Leclercia sp. J807]
MHVVSRAPFEMATRRFPNQALALDDLYRVLKKENYTSPNQMRKRFPSLDRMKYREKWWVIDVGGGHLRVMFFADFARGRI
FIKHITTHAEYDKLTDIYRRTKE

Nucleotide

• Length: 312 bp

>T144300 NZ_CP061842:3825972-3826190 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15442.58 Da
• Isoelectric Point: 4.7657

>AT144300 WP_156237978.1 NZ_CP046446:c1138110-1137691 [Leclercia sp. J807]
MKYAEAINAANNLTNVLPFLGARPSRKDYEDALALVEYLLEHQPDSPLIDMLTAKIDKYESESPEFAEFNARIAGTPSGV
ALLRTLMDQYDLTQSDLENEIGKKSLVSRILNGQRTLTLDHMRALAKRFGLPVSAFVGD

Nucleotide

• Length: 420 bp

>AT144300 NZ_CP061842:3825571-3825945 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG