Detailed information of TA system TA144270

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2854341..2854972
• Replicon: chromosome
• Accession: NZ_CP046444
• Organism: Granulicella sp. 5B5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GOB94_RS12105
• Protein ID: WP_182276158.1
• Coordinates: 2854341..2854631 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GOB94_RS12110
• Protein ID: WP_182276159.1
• Coordinates: 2854646..2854972 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GOB94_RS12085	2849770..2850648	-	879	WP_182276155.1	follicular epithelium yolk protein subunit	-
GOB94_RS12090	2850861..2851451	-	591	WP_182276156.1	thiamine phosphate synthase	-
GOB94_RS12095	2851773..2853167	+	1395	WP_182276157.1	Si-specific NAD(P)(+) transhydrogenase	-
GOB94_RS12100	2853366..2854313	+	948	WP_182278603.1	ROK family protein	-
GOB94_RS12105	2854341..2854631	+	291	WP_182276158.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GOB94_RS12110	2854646..2854972	+	327	WP_182276159.1	HigA family addiction module antidote protein	Antitoxin
GOB94_RS12115	2854969..2855790	-	822	WP_182276160.1	bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase	-
GOB94_RS12120	2856170..2857582	+	1413	WP_182276161.1	lipopolysaccharide biosynthesis protein	-
GOB94_RS12125	2857680..2857856	+	177	WP_182276162.1	hypothetical protein	-
GOB94_RS12130	2857891..2858400	+	510	WP_182276163.1	DUF1572 family protein	-
GOB94_RS12135	2858496..2859842	+	1347	WP_182276164.1	adenylosuccinate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2846792..2858400	11608

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11775.24 Da
• Isoelectric Point: 5.9052

>T144270 WP_182276158.1 NZ_CP046444:2854341-2854631 [Granulicella sp. 5B5]
MIESWRDAWLREFFVNDTYSKELPSELRDRVFRKLQMIDDATCDADLRVPPSNRFERLSGRLEGFRSIRVNERWRLIFKW
DGSDVRDIYLDDHSYR

Nucleotide

• Length: 291 bp

>T144270 NZ_CP061802:c125976-125518 [Enterobacter mori]
GTGATCCTTTACCGACTGACCAAGACCCGTTATCTGATGACTGCGTGGTCTGGTCTGGGTGCGAAAGAAGCGGGGGGCCG
CTGGAACAGCGTAGGCACTGCAATGGTCTACCTGTCTGAAACGGCCAGCCTTACCATGCTTGAAACGCTGGTACACATTC
ACGCGCCGCAGTTACTGGACGACTTTACACTGCTGAGTCTTGATGTACCGGATGATCAGATCCAGACCTTCGACATGAGC
CGTCTCCCTGATAACTGGGCGAGTGAGGATGCTCCTGCTGAGCTGGCGCTCTACGGTGATAACTGGGCTGAAAGCGGTTC
TTCTATCGCGCTGCGTATTCCAAGTGCGTTGTCTCCGGTTGAATTTAACTACTTGCTGAATCCGGCACACCCTGAACTCT
TTGACCTGATGGCTACGGTTAAGAAAATTCCGTTCCGGTTTGATTCCCGCCTGAAATAA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12289.24 Da
• Isoelectric Point: 11.3938

>AT144270 WP_182276159.1 NZ_CP046444:2854646-2854972 [Granulicella sp. 5B5]
MLLTKRRPVSVGEILTEEFLEPLGLTQSEFAVRTGLPRKHVNELCRDRRAVTADTALILARVFGNSADFWLNTQRRMDLW
EALNTPKRAERIARATPLNMKSKRANAA

Nucleotide

• Length: 327 bp

>AT144270 NZ_CP061802:c126419-125973 [Enterobacter mori]
ATGACGATGAAAGTTTTTTCTCCAGACGTTACCAAAACGAATCAGCACTGCTTATGGCAGGTTGCTGGGCTGGATAATGA
CGGTATCGCGCTGATGGATAAAATCAATCACGGTCTCGATGGCACAGTAGCTAAGCACATTTCTGAATGGGCTAACATCA
CGCCGTCTGAGCTGCGCAAAATGTCGGGTATACCTAATACGACGTTTAATCGTAGCATCAAAGACCGTTTCACTGCCGAT
CAGAGCGAACGTCTGGTGCGCATTATCCGCGTAATTGAACGCGCTGTAGAGCTGTTTGAAGGTGACAAAGAAGCGGCGCA
CAAATGGCTGAACGAAGCGAACCGTGGTCTGAGCTGGAAATCACCGGCAGAGCTTGTTTCTTCTGAGACAGGTGCGCTTG
AAGTGATGCGCCTGATTACGCGTATTGAGCACGGGGTATACTCGTGA