Detailed information of TA system TA144269

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2700601..2701191
• Replicon: chromosome
• Accession: NZ_CP046444
• Organism: Granulicella sp. 5B5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GOB94_RS11450
• Protein ID: WP_182276039.1
• Coordinates: 2700907..2701191 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: GOB94_RS11445
• Protein ID: WP_182276038.1
• Coordinates: 2700601..2700891 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GOB94_RS11435	2696070..2699204	+	3135	WP_182276036.1	DNA polymerase I	-
GOB94_RS11440	2699223..2700287	-	1065	WP_182276037.1	RelA/SpoT domain-containing protein	-
GOB94_RS11445	2700601..2700891	-	291	WP_182276038.1	HigA family addiction module antidote protein	Antitoxin
GOB94_RS11450	2700907..2701191	-	285	WP_182276039.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GOB94_RS11455	2701503..2702843	-	1341	WP_182276040.1	phosphoglucosamine mutase	-
GOB94_RS11460	2702886..2704382	-	1497	WP_182276041.1	exodeoxyribonuclease VII large subunit	-
GOB94_RS11465	2704429..2705646	+	1218	WP_182276042.1	potassium channel family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-94)

Antitoxin

• Cro/C1-type helix-turn-helix domain (7-64)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10754.32 Da
• Isoelectric Point: 9.7113

>T144269 WP_182276039.1 NZ_CP046444:c2701191-2700907 [Granulicella sp. 5B5]
MILSCRDKRTSEFVAGKMVKAFSGFERAAQMKIDRMQAATSINDLAALPGNRFEALKGDRKGQYSIRINDQWRICFKWPQ
GERGPSDVEIVDYH

Nucleotide

• Length: 285 bp

>T144269 NZ_CP061802:118461-118769 [Enterobacter mori]
ATGCAATATACCGTTTACCGCAATCCCGGCAACAGCCAGGCATACCCTTACCTGCTGGATATACAGAGCGACATCATTGG
CGAGTTAAATACCCGCCTGGTGATCCCCCTTCATCGGCTTAAAAAAGGAGCCTCGGCCCCGGTTGCGCGATTAACTCCGG
TCATACAGGTGGAAGGGAATGACGTTATCCTGATGACGCATGAAATGGCTTCAGTCCGCGTGAAGCAGCTCGGTCAGGCG
GTCATGGACGCATCTCCGTTCCGGCACACTATCAAAAGTGCGGTGGACTTCCTGCTGGATGGTTTCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10682.41 Da
• Isoelectric Point: 9.8632

>AT144269 WP_182276038.1 NZ_CP046444:c2700891-2700601 [Granulicella sp. 5B5]
MGPIHPGEQLAEELEEMGLSAAAFARQIQVPTNRVTEILKGRRSITGDTALRLAHFFGTSAEFWLNLQSLYEIRVAEKKF
GKMIKGLPTIKDLRAA

Nucleotide

• Length: 291 bp

>AT144269 NZ_CP061802:118219-118458 [Enterobacter mori]
ATGCAGGCTACAGCTGCTCGCCAGAAAAAAACCGTCAGTGTCACCCTTGAGCCAATGCTCGTACAGCAGGCAAAAGACGC
TGGGATTAACTTGTCCGCCACGCTGGCAGAAGCGCTTCAGAGCAAACTGAAGGCCAGTGCAGCGGAAGAATGGAAACGAC
AGAATCGTGCTGGTATTCAGGAGTTGAACCGCATCACTGAAGAGCATGGGTTACTGTCTGACGAATACAGGACGTTCTGA