Detailed information of TA system TA144264

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 610032..610675
• Replicon: chromosome
• Accession: NZ_CP046444
• Organism: Granulicella sp. 5B5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GOB94_RS02690
• Protein ID: WP_182277387.1
• Coordinates: 610032..610355 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GOB94_RS02695
• Protein ID: WP_182277388.1
• Coordinates: 610352..610675 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GOB94_RS02660	605196..605873	+	678	WP_182277383.1	hypothetical protein	-
GOB94_RS02665	605994..607430	+	1437	WP_182277384.1	hypothetical protein	-
GOB94_RS02670	607601..608251	+	651	WP_182277385.1	DinB family protein	-
GOB94_RS02675	608474..608641	-	168	WP_182277386.1	hypothetical protein	-
GOB94_RS02680	608686..609042	-	357	WP_182278389.1	hypothetical protein	-
GOB94_RS02685	609064..609651	-	588	WP_182278390.1	sigma-70 family RNA polymerase sigma factor	-
GOB94_RS02690	610032..610355	+	324	WP_182277387.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GOB94_RS02695	610352..610675	+	324	WP_182277388.1	helix-turn-helix domain-containing protein	Antitoxin
GOB94_RS02700	611573..613246	-	1674	WP_182277389.1	FAD-dependent oxidoreductase	-
GOB94_RS02705	613491..615107	+	1617	WP_182277390.1	B12-binding domain-containing radical SAM protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	599804..610675	10871

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-85)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12542.75 Da
• Isoelectric Point: 10.4037

>T144264 WP_182277387.1 NZ_CP046444:610032-610355 [Granulicella sp. 5B5]
MSWIVEFLDEDVKVLFDSLPLDMRAHFQRIVELIQSHGLERVREPYVKHLEGALWEMRMKGRSGIARAVYVTAIGKRIVV
VHVFTKKTQKTPRREIETALKRAKEVE

Nucleotide

• Length: 324 bp

>T144264 NZ_CP061801:c2687955-2687851 [Enterobacter mori]
GGCAAGGCGATTTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATA
CAGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12073.84 Da
• Isoelectric Point: 10.9913

>AT144264 WP_182277388.1 NZ_CP046444:610352-610675 [Granulicella sp. 5B5]
MKRKFIPVEDSFAQWKKDPKYAAAYDALEEEFAFASSLIKARTAADMTQAQVAEAMGTTQAVVARLESGRVRPSTRTLER
FAKATHSRLRIIFEPQVRPARSRANTK

Nucleotide

• Length: 324 bp

>AT144264 NZ_CP061801:2687846-2687990 [Enterobacter mori]
CAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTT
GGCATTAATGCAGGCTAAAATCGCCTTGCCTTTTAAGAATAGATGACGACGCCAGGTTTTCCAGT