Detailed information of TA system TA144254

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 72224..72650
• Replicon: plasmid pS4-aph
• Accession: NZ_CP046417
• Organism: Escherichia coli strain LZYWN_4

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: GJV83_RS23325
• Protein ID: WP_001312861.1
• Coordinates: 72224..72382 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	72426..72650 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJV83_RS23300	67592..68281	-	690	WP_039002187.1	conjugal transfer transcriptional regulator TraJ	-
GJV83_RS23305	68468..68851	-	384	WP_001151524.1	relaxosome protein TraM	-
GJV83_RS23310	69172..69774	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
GJV83_RS23315	70071..70892	-	822	WP_001234469.1	DUF945 domain-containing protein	-
GJV83_RS23320	71015..71302	-	288	WP_000107535.1	hypothetical protein	-
GJV83_RS23325	72224..72382	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	72426..72650	-	225	NuclAT_0	-	Antitoxin
-	72426..72650	-	225	NuclAT_0	-	Antitoxin
-	72426..72650	-	225	NuclAT_0	-	Antitoxin
-	72426..72650	-	225	NuclAT_0	-	Antitoxin
GJV83_RS23330	72462..72650	+	189	WP_001299721.1	hypothetical protein	-
GJV83_RS23335	72662..73381	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
GJV83_RS23340	73378..73812	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
GJV83_RS23345	73867..75825	-	1959	WP_039000998.1	ParB/RepB/Spo0J family partition protein	-
GJV83_RS23350	75890..76123	-	234	WP_000005987.1	DUF905 family protein	-
GJV83_RS23355	76180..76719	-	540	WP_032332913.1	single-stranded DNA-binding protein	-
GJV83_RS23360	76745..76951	-	207	WP_000275853.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(M) / sul3 / aph(4)-Ia / aac(3)-IVa / blaTEM-1B	-	1..113995	113995

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T144254 WP_001312861.1 NZ_CP046417:c72382-72224 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T144254 NZ_CP061800:5471775-5472056 [Desulfonema magnum]
ATGATTAAAACATTCAAACAGAATTGGCTTGAAGCTTTTTTTCATGGAGGAAAACACAAGCGGGTTCCTTCTCAGTTGAA
ACGTCAGTTGAGACATAAGCTTGATATGCTGAATTCAGCTTCAGTGCTGAGAGATCTGGAATCACCACCGGGAAATCATC
TTCATCCATTGAGCAGGGAGCGTGAAGGAGAGTATGCGATTTCTGTCAATGGTCCGTGGAGACTCTGTTTCCGATTTGAA
AACGGTGATGCCTATGACGTGGAACTCGTACAATATCATTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT144254 NZ_CP046417:c72650-72426 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin