Detailed information of TA system TA144250

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 36942..37196
• Replicon: plasmid pS4-aph
• Accession: NZ_CP046417
• Organism: Escherichia coli strain LZYWN_4

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: GJV83_RS23145
• Protein ID: WP_001351576.1
• Coordinates: 36942..37148 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	37135..37196 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJV83_RS23090	32067..32774	-	708	WP_058100829.1	hypothetical protein	-
GJV83_RS23095	32776..33033	-	258	WP_001685860.1	hypothetical protein	-
GJV83_RS23100	33129..33406	-	278	Protein_41	type II toxin-antitoxin system RelE/ParE family toxin	-
GJV83_RS23105	33406..33690	-	285	WP_001407321.1	ribbon-helix-helix domain-containing protein	-
GJV83_RS23110	33779..33841	-	63	Protein_43	3'-5' exonuclease	-
GJV83_RS23115	33876..34083	-	208	Protein_44	type II toxin-antitoxin system RelE/ParE family toxin	-
GJV83_RS23120	34080..34349	-	270	WP_021559054.1	hypothetical protein	-
GJV83_RS23125	34404..34562	-	159	WP_072649723.1	hypothetical protein	-
GJV83_RS23130	35261..36118	-	858	WP_039002220.1	incFII family plasmid replication initiator RepA	-
GJV83_RS23135	36111..36185	-	75	WP_061357229.1	RepA leader peptide Tap	-
GJV83_RS23140	36417..36674	-	258	WP_001541895.1	replication regulatory protein RepA	-
GJV83_RS23145	36942..37148	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	37135..37196	+	62	NuclAT_1	-	Antitoxin
-	37135..37196	+	62	NuclAT_1	-	Antitoxin
-	37135..37196	+	62	NuclAT_1	-	Antitoxin
-	37135..37196	+	62	NuclAT_1	-	Antitoxin
GJV83_RS23150	37538..37750	-	213	WP_001541896.1	ANR family transcriptional regulator	-
GJV83_RS23155	37885..38445	-	561	WP_000139310.1	fertility inhibition protein FinO	-
GJV83_RS23160	38500..39243	-	744	WP_039002216.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(M) / sul3 / aph(4)-Ia / aac(3)-IVa / blaTEM-1B	-	1..113995	113995

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T144250 WP_001351576.1 NZ_CP046417:c37148-36942 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T144250 NZ_CP061800:3066467-3066721 [Desulfonema magnum]
ATGATGATTGCATGGACTGACCGGGCTTGGAACGAATACTTGTACTGGCAAAAACATGACAAAAAAATTGTTAAACGGAT
CAATGAATTGATACGAGATATCAAACGTCATCCTGAAGAAGGCATTGGTAAACCGGAAGCACTTCGTTTTGATTTGTCAG
GAAAATGGTCGCGCAGAATCAATCAGGAGCATAGACTGGTGTACCAAGTCATTGATGAGATGATAGTAATTTTTCAGTGT
CGTTATCATTATTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT144250 NZ_CP046417:37135-37196 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin