Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3740000..3740541 Replicon chromosome
Accession NZ_CP046416
Organism Escherichia coli strain LZYWN_4

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag GJV83_RS18245 Protein ID WP_000615983.1
Coordinates 3740263..3740541 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag GJV83_RS18240 Protein ID WP_000729703.1
Coordinates 3740000..3740260 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJV83_RS18225 3736051..3738144 + 2094 WP_001140826.1 flagellar biosynthesis protein FlhA -
GJV83_RS18230 3738368..3738865 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
GJV83_RS18235 3739041..3739790 - 750 WP_000056849.1 C40 family peptidase -
GJV83_RS18240 3740000..3740260 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
GJV83_RS18245 3740263..3740541 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
GJV83_RS18250 3740697..3741437 + 741 WP_001225679.1 murein L,D-transpeptidase -
GJV83_RS18255 3741408..3742175 - 768 WP_000333380.1 class II glutamine amidotransferase -
GJV83_RS18260 3742381..3742959 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA / rhs/PAAR / tssA / hcp1/tssD1 3739041..3761242 22201


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T144247 WP_000615983.1 NZ_CP046416:3740263-3740541 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T144247 NZ_CP061800:c1996925-1996602 [Desulfonema magnum]
ATGGTAGTCAGCAGATTCGATGTATGTCTTGTCAATCTTGATCCCACTGTCGGGTCCGAGATAAAAAAAGTCAGACCATG
TCTGGTCATTTCCCCGGACGAGATGAATCATAATATACGCACGGTAATTGTCGCCCCCATGACCACGGCCGGAAAAAATT
ACCCCACGCGGGTTCAATGCATGTTCAAAAGAAAAAAGGGACAGATTGTTCTTGATCAGATACGCACTGTTGACAAAACC
CGAGTCATTAAAAAGCTCGGCGTTATTGATCTGAAAACTCAGACAGAAGTTATCTCCGTCTTACAGCAGTTATTTTCGTT
CTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT144247 WP_000729703.1 NZ_CP046416:3740000-3740260 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT144247 NZ_CP061800:c1997173-1996919 [Desulfonema magnum]
ATGAGAGCGCGTATTATAAAAATTGGCAATTCGCAGGGCATCAGAATTCCGAAGCCCTTGCTGGATCAGAGCGGGATAAG
CACGGATGTCGAGTTGGAAGTGGAGAAGCATCGGATAATCATTCGCCCGGTCCCGGATGTGAGAGCCGGATGGGACAATG
CCTTCAAACAGATGGCTCAGAATGGCGATGATTCCCTTGAAAGCGGTGCGGATGTCATCTCGCATTCATGGGATGAGGAT
GAATGGGAATGGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References