Detailed information of TA system TA144246

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3698221..3698915
• Replicon: chromosome
• Accession: NZ_CP046416
• Organism: Escherichia coli strain LZYWN_4

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: GJV83_RS18005
• Protein ID: WP_001263489.1
• Coordinates: 3698221..3698619 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: GJV83_RS18010
• Protein ID: WP_000554758.1
• Coordinates: 3698622..3698915 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	3693809..3693889	-	81	NuclAT_11	-	-
-	3693809..3693889	-	81	NuclAT_11	-	-
-	3693809..3693889	-	81	NuclAT_11	-	-
-	3693809..3693889	-	81	NuclAT_11	-	-
GJV83_RS17980	3694485..3694943	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
GJV83_RS17985	3695204..3696661	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
GJV83_RS17990	3696718..3697239	-	522	Protein_3521	peptide chain release factor H	-
GJV83_RS17995	3697235..3697441	-	207	Protein_3522	RtcB family protein	-
GJV83_RS18000	3697759..3698211	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
GJV83_RS18005	3698221..3698619	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
GJV83_RS18010	3698622..3698915	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
GJV83_RS18015	3698967..3700022	-	1056	WP_001226164.1	DNA polymerase IV	-
GJV83_RS18020	3700093..3701016	-	924	WP_001232546.1	putative lateral flagellar export/assembly protein LafU	-
GJV83_RS18025	3701019..3701882	-	864	WP_001169518.1	flagellar motor stator protein MotA	-
GJV83_RS18030	3701895..3702611	-	717	WP_000938723.1	FliA/WhiG family RNA polymerase sigma factor	-
GJV83_RS18035	3702631..3703098	-	468	WP_000725257.1	flagellar basal body-associated FliL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T144246 WP_001263489.1 NZ_CP046416:c3698619-3698221 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T144246 NZ_CP061800:c1851902-1851648 [Desulfonema magnum]
ATGAATAAAAAACAAAGAAGGACATTGGAAAAAATTTTTGATAATCCGGTTCAGTCAAATATCGAGTGGAACGATATTGA
AAAAATGCTCATTGCACTGGGGGCTGAAATCAGCGAAGGCAGAGGATCAAGAATCCGTATTGCTCTTAACAATGTCAGAG
CCGTATTTCACCGCCCTCATCCGAGAAAAGAAACCGATAAAGGAGCAGTTGTTTCCATGCGTCGCTTCCTTACAGAAGCA
GGAATTACACTATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT144246 WP_000554758.1 NZ_CP046416:c3698915-3698622 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT144246 NZ_CP061800:c1851651-1851325 [Desulfonema magnum]
ATGAAATATAAAGAATATATTGCAAAAGTCGAGTATGATGATGAAGCGGATATTTTTCACGGTGATGTTATAAATATCCG
TGATGTCATAACTTTTCAAGGCAAATCGGTCGAAGAATTAAAAGAGGCTTTTATTGATTCAGTTGAGGATTATATTGAAT
TTTGCTCGGAAAGAGGAGAAGAGCCTGACAAACCGTTTAGCGGAAGCTTTACAATTAATCTGCCGCCTGATCTTCATAAA
AAAATTGTTCTTGCAATTGAAAAGTCAGATAAAAATTTAGATAATTGGGTTAAAGATGTCTTTGAAAATGCGGTAAGCAG
CCATTAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3