Detailed information of TA system TA144210

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5557326..5557921
• Replicon: chromosome
• Accession: NZ_CP046405
• Organism: Pseudomonas aeruginosa strain SE5443

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: GNT61_RS25885
• Protein ID: WP_003113526.1
• Coordinates: 5557643..5557921 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GNT61_RS25880
• Protein ID: WP_003099268.1
• Coordinates: 5557326..5557631 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GNT61_RS25850	5552370..5552642	-	273	WP_003115921.1	hypothetical protein	-
GNT61_RS25855	5553089..5553424	+	336	WP_073648965.1	hypothetical protein	-
GNT61_RS25860	5553434..5554987	-	1554	WP_179063487.1	SIR2 family protein	-
GNT61_RS25865	5555007..5555378	-	372	WP_073648964.1	ASCH domain-containing protein	-
GNT61_RS25870	5555356..5556408	-	1053	WP_073648963.1	N-acetyltransferase	-
GNT61_RS25875	5556427..5556948	-	522	WP_179063474.1	AAA family ATPase	-
GNT61_RS25880	5557326..5557631	-	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
GNT61_RS25885	5557643..5557921	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GNT61_RS25890	5558250..5560478	+	2229	WP_003113525.1	TonB-dependent receptor	-
GNT61_RS25895	5560548..5561195	-	648	WP_003095021.1	carbonate dehydratase	-
GNT61_RS25900	5561257..5562495	-	1239	WP_003123433.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T144210 WP_003113526.1 NZ_CP046405:c5557921-5557643 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T144210 NZ_CP061778:c209970-209671 [Pseudomonas aeruginosa]
ATGGTTGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT144210 WP_003099268.1 NZ_CP046405:c5557631-5557326 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT144210 NZ_CP061778:c209674-209384 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
CTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCTCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCGACATTGAAAACCATGCTGGCA
GTAATGCGAGCTTTGGGCGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Structures

Toxin

• AlphaFold DB: V6ALY3