Detailed information of TA system TA144196

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 6123969..6124564
• Replicon: chromosome
• Accession: NZ_CP046403
• Organism: Pseudomonas aeruginosa strain SE5369

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: GNT46_RS28985
• Protein ID: WP_003117425.1
• Coordinates: 6124286..6124564 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GNT46_RS28980
• Protein ID: WP_003099268.1
• Coordinates: 6123969..6124274 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GNT46_RS28945	6119422..6119712	-	291	WP_023083237.1	DUF5447 family protein	-
GNT46_RS28950	6119716..6119931	-	216	WP_031629304.1	hypothetical protein	-
GNT46_RS28955	6119924..6120196	-	273	WP_003115921.1	hypothetical protein	-
GNT46_RS28960	6120306..6120572	+	267	WP_016852153.1	hypothetical protein	-
GNT46_RS28965	6120746..6121540	+	795	WP_023082695.1	helix-turn-helix domain-containing protein	-
GNT46_RS28970	6121515..6123053	+	1539	WP_023082696.1	GNAT family N-acetyltransferase	-
GNT46_RS28975	6123065..6123592	-	528	WP_071535723.1	AAA family ATPase	-
GNT46_RS28980	6123969..6124274	-	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
GNT46_RS28985	6124286..6124564	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GNT46_RS28990	6124893..6127121	+	2229	WP_003099265.1	TonB-dependent receptor	-
GNT46_RS28995	6127191..6127838	-	648	WP_003095021.1	carbonate dehydratase	-
GNT46_RS29000	6127900..6129138	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T144196 WP_003117425.1 NZ_CP046403:c6124564-6124286 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T144196 NZ_CP061764:4009814-4010191 [Escherichia coli O19:H7]
ATGCAAACCCAACCACTATCCTCAACGCAGGAGGCTACATCACGCCCGTCACCGGTGGAGATCTGGCAACGGCTTCTGAG
CCACCTGTTGGATCGTCACTACGGTCTGACGCTTAACGACACGCCGTTTGGCAACGACGGTGTGATTCAGGAGCATATCG
ACGCCGGTATATCACTGTGCGACGCCGTGAATTTTATTGTGGAGAAGTACGATCTGGTGCGCATCGATCGTCACGGTTTC
AGTACAGAAACACAGTTGCCGCGTCTCACCAGTATCGATATTCTCCGCGCCCGCAAAGCCACCGGGTTGATGACTCGCAA
CGATTACAGAACGGTGACCGACATCACCACCGGTAAATACCGTGGGGGGCATCGATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT144196 WP_003099268.1 NZ_CP046403:c6124274-6123969 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT144196 NZ_CP061764:4009404-4009763 [Escherichia coli O19:H7]
TTGTCGAATAAAATACCGACAGTAAACCACGATATCACTGAACCCTGGTGGGGGCTTAAACGCAGTATCACGCCCTGTTT
TGGCGCACGGCTGGTGCAGTCGGGCAACTGTCTGCATTATCTCGCTGACCGGGCCAGCATCACCGGACAGTTCAGCGACG
GGGATTTACGGCATCTCGACCAGGCATTTCCACTGCTGCTCAAACAGCTTGAACTGATGCTCACCAGCGGTGAACTCACT
CCCCGCCATCAGCACTGTGTCACGCTCTATGCAAAAGGGCTGACCTGCGAGGCCGACTCCCTCGGCTCGCATGGTTACGT
ATACCTGGCGATTTATCCGACACCAGCGGCAACCGCGTAA

Structures

Toxin

• AlphaFold DB: A0A241XLJ5