Detailed information of TA system TA144188

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 6003817..6004412
• Replicon: chromosome
• Accession: NZ_CP046402
• Organism: Pseudomonas aeruginosa strain SE5331

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: GNT44_RS28390
• Protein ID: WP_003113526.1
• Coordinates: 6004134..6004412 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GNT44_RS28385
• Protein ID: WP_003133769.1
• Coordinates: 6003817..6004122 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GNT44_RS28370	6000523..6001386	-	864	WP_031761182.1	integrase domain-containing protein	-
GNT44_RS28375	6001987..6003144	-	1158	WP_031761183.1	hypothetical protein	-
GNT44_RS28385	6003817..6004122	-	306	WP_003133769.1	HigA family addiction module antidote protein	Antitoxin
GNT44_RS28390	6004134..6004412	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GNT44_RS28395	6004741..6006969	+	2229	WP_023104079.1	TonB-dependent receptor	-
GNT44_RS28400	6007040..6007687	-	648	WP_003095021.1	carbonate dehydratase	-
GNT44_RS28405	6007749..6008987	-	1239	WP_003095023.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T144188 WP_003113526.1 NZ_CP046402:c6004412-6004134 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T144188 NZ_CP061764:1932285-1932539 [Escherichia coli O19:H7]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11214.73 Da
• Isoelectric Point: 4.6081

>AT144188 WP_003133769.1 NZ_CP046402:c6004122-6003817 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT144188 NZ_CP061764:1932037-1932288 [Escherichia coli O19:H7]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: V6ALY3