Detailed information of TA system TA144179

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4836946..4837548
• Replicon: chromosome
• Accession: NZ_CP046396
• Organism: Escherichia coli strain ampC_0069

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: GNX12_RS23130
• Protein ID: WP_000897305.1
• Coordinates: 4837237..4837548 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GNX12_RS23125
• Protein ID: WP_000356397.1
• Coordinates: 4836946..4837236 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GNX12_RS23105	4833448..4834350	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
GNX12_RS23110	4834347..4834982	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
GNX12_RS23115	4834979..4835908	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
GNX12_RS23120	4836123..4836341	-	219	WP_001298592.1	ribbon-helix-helix domain-containing protein	-
GNX12_RS23125	4836946..4837236	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
GNX12_RS23130	4837237..4837548	-	312	WP_000897305.1	hypothetical protein	Toxin
GNX12_RS23135	4837777..4838685	+	909	WP_001318161.1	alpha/beta hydrolase	-
GNX12_RS23140	4838749..4839690	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
GNX12_RS23145	4839735..4840172	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
GNX12_RS23150	4840169..4841041	-	873	WP_000920762.1	virulence factor BrkB family protein	-
GNX12_RS23155	4841035..4841634	-	600	WP_001314324.1	glucose-1-phosphatase	-
GNX12_RS23160	4841733..4842518	-	786	WP_000059679.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T144179 WP_000897305.1 NZ_CP046396:c4837548-4837237 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T144179 NZ_CP061760:5430-5735 [Escherichia coli O68:H12]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT144179 WP_000356397.1 NZ_CP046396:c4837236-4836946 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT144179 NZ_CP061760:5210-5428 [Escherichia coli O68:H12]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGCCTGCGTGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7