Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3946962..3947656 Replicon chromosome
Accession NZ_CP046396
Organism Escherichia coli strain ampC_0069

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag GNX12_RS18840 Protein ID WP_001263491.1
Coordinates 3946962..3947360 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag GNX12_RS18845 Protein ID WP_000554755.1
Coordinates 3947363..3947656 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GNX12_RS18810 3942224..3943681 + 1458 WP_022645227.1 cytosol nonspecific dipeptidase -
GNX12_RS18815 3943690..3943971 + 282 WP_022645226.1 hypothetical protein -
GNX12_RS18820 3943988..3944497 - 510 WP_001361775.1 hydrolase -
GNX12_RS18825 3944559..3945173 - 615 WP_022645225.1 peptide chain release factor H -
GNX12_RS18830 3945170..3946321 - 1152 WP_023909049.1 RNA ligase RtcB family protein -
GNX12_RS18835 3946500..3946952 - 453 WP_023909048.1 GNAT family N-acetyltransferase -
GNX12_RS18840 3946962..3947360 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
GNX12_RS18845 3947363..3947656 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
GNX12_RS18850 3947708..3948763 - 1056 WP_022645222.1 DNA polymerase IV -
GNX12_RS18855 3948834..3949619 - 786 WP_072224360.1 putative lateral flagellar export/assembly protein LafU -
GNX12_RS18860 3949564..3951303 + 1740 WP_022645220.1 flagellar biosynthesis protein FlhA -
GNX12_RS18865 3951401..3952174 - 774 WP_022645219.1 C40 family peptidase -
GNX12_RS18870 3952360..3952620 + 261 WP_000729708.1 type II toxin-antitoxin system antitoxin DinJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T144173 WP_001263491.1 NZ_CP046396:c3947360-3946962 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T144173 NZ_CP061758:2690055-2690162 [Escherichia coli O68:H12]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT144173 WP_000554755.1 NZ_CP046396:c3947656-3947363 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT144173 NZ_CP061758:c2690008-2689942 [Escherichia coli O68:H12]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References