Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 2706822..2707501 Replicon chromosome
Accession NZ_CP046396
Organism Escherichia coli strain ampC_0069

Toxin (Protein)


Gene name higB Uniprot ID A0A0A1A1R1
Locus tag GNX12_RS12845 Protein ID WP_000057541.1
Coordinates 2706822..2707124 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag GNX12_RS12850 Protein ID WP_000806442.1
Coordinates 2707160..2707501 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GNX12_RS12825 2702085..2703305 - 1221 WP_022645286.1 fosmidomycin MFS transporter -
GNX12_RS12830 2703523..2705175 + 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
GNX12_RS12835 2705212..2705691 - 480 WP_000186638.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
GNX12_RS12840 2705895..2706689 - 795 WP_022645287.1 TraB/GumN family protein -
GNX12_RS12845 2706822..2707124 + 303 WP_000057541.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GNX12_RS12850 2707160..2707501 + 342 WP_000806442.1 HigA family addiction module antidote protein Antitoxin
GNX12_RS12855 2707559..2710063 - 2505 WP_022645288.1 copper-exporting P-type ATPase CopA -
GNX12_RS12860 2710326..2711258 + 933 WP_022645289.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2706822..2716102 9280


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11795.37 Da        Isoelectric Point: 10.2638

>T144167 WP_000057541.1 NZ_CP046396:2706822-2707124 [Escherichia coli]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T144167 NZ_CP061758:1036753-1037088 [Escherichia coli O68:H12]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT144167 WP_000806442.1 NZ_CP046396:2707160-2707501 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT144167 NZ_CP061758:1036505-1036753 [Escherichia coli O68:H12]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A1A1R1

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References