Detailed information of TA system TA144153

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 633180..633979
• Replicon: chromosome
• Accession: NZ_CP046396
• Organism: Escherichia coli strain ampC_0069

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: GNX12_RS02980
• Protein ID: WP_000347273.1
• Coordinates: 633180..633644 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: D6JF08
• Locus tag: GNX12_RS02985
• Protein ID: WP_001308975.1
• Coordinates: 633644..633979 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GNX12_RS02950	628181..628615	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
GNX12_RS02955	628633..629511	-	879	WP_001298314.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
GNX12_RS02960	629501..630280	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
GNX12_RS02965	630291..630764	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
GNX12_RS02970	630787..632067	-	1281	WP_023908831.1	tagatose-bisphosphate aldolase subunit KbaZ	-
GNX12_RS02975	632316..633125	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
GNX12_RS02980	633180..633644	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
GNX12_RS02985	633644..633979	-	336	WP_001308975.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
GNX12_RS02990	634128..635699	-	1572	WP_023908830.1	galactarate dehydratase	-
GNX12_RS02995	636074..637408	+	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
GNX12_RS03000	637424..638194	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T144153 WP_000347273.1 NZ_CP046396:c633644-633180 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T144153 NZ_CP061754:c2623821-2623645 [Escherichia coli O18ac:H14]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12343.97 Da
• Isoelectric Point: 4.8616

>AT144153 WP_001308975.1 NZ_CP046396:c633979-633644 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT144153 NZ_CP061754:c2623599-2623183 [Escherichia coli O18ac:H14]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: D6JF08