Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3893099..3893696 Replicon chromosome
Accession NZ_CP046381
Organism Klebsiella pneumoniae strain BD_DM_782

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag GM011_RS18795 Protein ID WP_004142563.1
Coordinates 3893379..3893696 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag GM011_RS18790 Protein ID WP_004142561.1
Coordinates 3893099..3893386 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GM011_RS18760 3889179..3889427 + 249 WP_020325243.1 DUF1158 domain-containing protein -
GM011_RS18765 3889445..3889786 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
GM011_RS18770 3889817..3890932 - 1116 WP_020325248.1 MBL fold metallo-hydrolase -
GM011_RS18775 3891112..3891693 + 582 WP_020325240.1 TetR/AcrR family transcriptional regulator -
GM011_RS18780 3891693..3892061 + 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
GM011_RS18785 3892181..3892834 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
GM011_RS18790 3893099..3893386 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
GM011_RS18795 3893379..3893696 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GM011_RS18800 3893881..3894924 - 1044 WP_020325241.1 DUF2157 domain-containing protein -
GM011_RS18805 3895594..3896460 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
GM011_RS18810 3896569..3897996 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T144141 WP_004142563.1 NZ_CP046381:c3893696-3893379 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T144141 NZ_CP061750:90583-90741 [Escherichia coli O15:H12]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT144141 WP_004142561.1 NZ_CP046381:c3893386-3893099 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT144141 NZ_CP061750:90472-90537 [Escherichia coli O15:H12]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References