Detailed information of TA system TA144113

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 1518921..1519775
• Replicon: chromosome
• Accession: NZ_CP046377
• Organism: Pectobacterium polaris strain PZ1

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: A0A6P1S1D5
• Locus tag: GMX10_RS07030
• Protein ID: WP_039487843.1
• Coordinates: 1518921..1519409 (-)
• Length: 163 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: A0A6P1S4C9
• Locus tag: GMX10_RS07035
• Protein ID: WP_039487859.1
• Coordinates: 1519434..1519775 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GMX10_RS07010	1515444..1516112	+	669	WP_039487849.1	6-phosphogluconate phosphatase	-
GMX10_RS07015	1516132..1516860	-	729	WP_039487847.1	amino acid ABC transporter permease	-
GMX10_RS07020	1516862..1517605	-	744	WP_039487846.1	amino acid ABC transporter permease	-
GMX10_RS07025	1517886..1518722	-	837	WP_039487845.1	ABC transporter substrate-binding protein	-
GMX10_RS07030	1518921..1519409	-	489	WP_039487843.1	type II toxin-antitoxin system YhaV family toxin	Toxin
GMX10_RS07035	1519434..1519775	-	342	WP_039487859.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
GMX10_RS07040	1520108..1521286	-	1179	WP_039487841.1	benzoate/H(+) symporter BenE family transporter	-
GMX10_RS07045	1521378..1521947	+	570	WP_161546729.1	XRE family transcriptional regulator	-
GMX10_RS07050	1522008..1522739	-	732	WP_039487837.1	phosphate signaling complex protein PhoU	-
GMX10_RS07055	1522752..1523525	-	774	WP_039487836.1	phosphate ABC transporter ATP-binding protein PstB	-
GMX10_RS07060	1523612..1524499	-	888	WP_039275952.1	phosphate ABC transporter permease PstA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-148)

Antitoxin

• PrlF antitoxin (15-113)

Sequences

Toxin

Protein

• Length: 163 a.a.
• Molecular weight: 18690.49 Da
• Isoelectric Point: 6.8563

>T144113 WP_039487843.1 NZ_CP046377:c1519409-1518921 [Pectobacterium polaris]
MEYLDVNGWKICFHSCFLQQIAELAQKVVELKAAKPDEYLGKKETKLLHAIERVIEEWIAVDPLNPQYRQGDTLGDEFKH
WFRAKFLSQFRLFYRCSAEHKTIIIGWVNDFDTLRAYGSKTDAYKVFAGMLRAGEPPDDWEKLLNEAKAITATTSIPGFL
GK

Nucleotide

• Length: 489 bp

>T144113 NZ_CP061749:708782-709096 [Escherichia coli O15:H12]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12567.24 Da
• Isoelectric Point: 4.4335

>AT144113 WP_039487859.1 NZ_CP046377:c1519775-1519434 [Pectobacterium polaris]
MAIQINTADVSLRAESRLTERSQTTIPAAIRDALHLKPGEYIKYTLLAGGKVIMSRQEEEQDDPVVSQFLTFLENDMKKN
PQNIHSIPVAFWESVKALTAGVEVDLDAPLTDD

Nucleotide

• Length: 342 bp

>AT144113 NZ_CP061749:709093-709509 [Escherichia coli O15:H12]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCGGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCAAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: A0A6P1S1D5

Antitoxin

• AlphaFold DB: A0A6P1S4C9