Detailed information of TA system TA144111

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1071908..1072632
• Replicon: chromosome
• Accession: NZ_CP046377
• Organism: Pectobacterium polaris strain PZ1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A093VC08
• Locus tag: GMX10_RS05035
• Protein ID: WP_039482109.1
• Coordinates: 1072321..1072632 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GMX10_RS05030
• Protein ID: WP_039482106.1
• Coordinates: 1071908..1072324 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GMX10_RS05005	1067403..1068644	+	1242	Protein_976	MFS transporter	-
GMX10_RS05010	1068713..1070065	+	1353	WP_039512786.1	aspartate aminotransferase family protein	-
GMX10_RS05015	1070159..1070617	-	459	WP_010286715.1	hypothetical protein	-
GMX10_RS05020	1071044..1071304	+	261	WP_039482487.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GMX10_RS05025	1071378..1071749	+	372	WP_039482489.1	helix-turn-helix domain-containing protein	-
GMX10_RS05030	1071908..1072324	-	417	WP_039482106.1	helix-turn-helix domain-containing protein	Antitoxin
GMX10_RS05035	1072321..1072632	-	312	WP_039482109.1	type II toxin-antitoxin system HigB family toxin	Toxin
GMX10_RS05040	1073634..1073927	-	294	WP_039482111.1	type I-E CRISPR-associated endoribonuclease Cas2	-
GMX10_RS05045	1073928..1074848	-	921	WP_039482113.1	type I-E CRISPR-associated endonuclease Cas1	-
GMX10_RS05050	1074873..1075520	-	648	WP_039482116.1	type I-E CRISPR-associated protein Cas6/Cse3/CasE	-
GMX10_RS05055	1075520..1076273	-	754	Protein_986	type I-E CRISPR-associated protein Cas5/CasD	-
GMX10_RS05060	1076283..1077350	-	1068	WP_039482121.1	type I-E CRISPR-associated protein Cas7/Cse4/CasC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1071378..1081010	9632

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12450.29 Da
• Isoelectric Point: 9.8530

>T144111 WP_039482109.1 NZ_CP046377:c1072632-1072321 [Pectobacterium polaris]
MHVISRAPFDTATTQFPNQAAALDDLYRVIKREMYATPDDMKKRFPSLDRMKYREKWWVIDIGGGHLRVMFFADFERGKI
FIKHITSHAEYDRLTEYYRRNKE

Nucleotide

• Length: 312 bp

>T144111 NZ_CP061749:328503-328778 [Escherichia coli O15:H12]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15375.68 Da
• Isoelectric Point: 4.8037

>AT144111 WP_039482106.1 NZ_CP046377:c1072324-1071908 [Pectobacterium polaris]
MMYADAIKAANNLTSIVPFLGGSASRKDYEDALKLTEYLIESEPDSPLIDMLTAKIDKYEDDAPEFAAFNERVKALPAGI
ALLRVLMDQHQLTQSDFEAEIGKKSLVSRILKGERTLTLDHMRALAKRFNIPVSAFVD

Nucleotide

• Length: 417 bp

>AT144111 NZ_CP061749:328775-329134 [Escherichia coli O15:H12]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: A0A093VC08