Detailed information of TA system TA144107

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 244664..245454
• Replicon: chromosome
• Accession: NZ_CP046377
• Organism: Pectobacterium polaris strain PZ1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GMX10_RS01215
• Protein ID: WP_161546601.1
• Coordinates: 245140..245454 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GMX10_RS01210
• Protein ID: WP_103161044.1
• Coordinates: 244664..245005 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GMX10_RS01170	240793..241452	+	660	WP_161546597.1	terminase endonuclease subunit	-
GMX10_RS01175	241544..242047	+	504	WP_103184670.1	head completion/stabilization protein	-
GMX10_RS01180	242047..242250	+	204	WP_039491308.1	tail protein X	-
GMX10_RS01185	242254..242547	+	294	WP_161546598.1	phage holin family protein	-
GMX10_RS01190	242534..243022	+	489	WP_161546599.1	glycoside hydrolase family 104 protein	-
GMX10_RS01195	243022..243468	+	447	WP_161547141.1	Rz-like lysis system protein LysB	-
GMX10_RS01200	243558..244013	+	456	WP_161546600.1	phage tail protein	-
GMX10_RS01205	244006..244635	+	630	WP_116156595.1	phage virion morphogenesis protein	-
GMX10_RS01210	244664..245005	-	342	WP_103161044.1	HigA family addiction module antidote protein	Antitoxin
GMX10_RS01215	245140..245454	-	315	WP_161546601.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GMX10_RS01220	246006..246326	+	321	Protein_230	KilA-N domain-containing protein	-
GMX10_RS01225	246345..246617	+	273	Protein_231	ORF6N domain-containing protein	-
GMX10_RS01230	246662..247720	-	1059	WP_103161047.1	hypothetical protein	-
GMX10_RS01235	247819..248454	+	636	WP_161546602.1	phage baseplate assembly protein V	-
GMX10_RS01240	248451..248795	+	345	WP_039512482.1	GPW/gp25 family protein	-
GMX10_RS01245	248799..249707	+	909	WP_161546603.1	baseplate assembly protein	-
GMX10_RS01250	249700..250311	+	612	WP_161546604.1	phage tail protein I	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	luxS	225573..269844	44271

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (24-70)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12244.05 Da
• Isoelectric Point: 9.9719

>T144107 WP_161546601.1 NZ_CP046377:c245454-245140 [Pectobacterium polaris]
MQKMRSIHSFRDQWLEDFFMYGKSHKKIPADVVTSLSRKLDIINAAVSAKDLRSPLGNRYENLNPPLGEYSSIRVNIQYR
LIFKWVDGKAEDIYLDDHGYKKHK

Nucleotide

• Length: 315 bp

>T144107 NZ_CP061744:c3702594-3702184 [Enterobacter hormaechei]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCACAGTGGATGTCCTTACTGCTCCACGGTCTGGTCGCGGC
AATGGTGTTATTGGTGCCGTGGCCGCTAAGCTATACCCCTCTGTGGCTGCTTTTGCTGTCGTTTGTGGTGTTTGATAGCG
TGCGCAGCCAGCGTCGAATCAACGCCCGTCAGGGTGAAATTAGGCTGCTGATGGATTCTCGCCTGCGCTGGCAGGGAAAA
GAGTGGGAGATGATGGGGACGCCGTGGATGCTTAACACCGGGATGATGCTACGGCTGCGTCGGGTCGAGGACAACCGTCG
CCAGCACCTGTGGCTGGCCGCAGACAGTATGGATCCCGCGGAGTGGCGGGATCTGCGCCGGCTAATCGTGCAACAGCCGA
CGCAGGAATAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12942.54 Da
• Isoelectric Point: 4.3501

>AT144107 WP_103161044.1 NZ_CP046377:c245005-244664 [Pectobacterium polaris]
MAQAQRKPTSVGDVLLYEYLEPTGLKINDLAEMLNVHRNTISALVNNNRKLTVDMAFRLAKAFDTSVDFWLNLQNSVDVW
EVENDARTQEEISRITTLEDFLAQRDENVKDVA

Nucleotide

• Length: 342 bp

>AT144107 NZ_CP061744:c3702841-3702575 [Enterobacter hormaechei]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATCTCCATCATGCCTTT
CTTCGAGTATGAGTACGACAGCTTAAGCGATGACGATAAGCGCCTGTTTGTCCGTCTGCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAATCACGGCAAGCCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CAGGAACGTGGTCCTGTGGCAATCTGA