Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3595224..3595942 Replicon chromosome
Accession NZ_CP046376
Organism Pectobacterium parmentieri strain HC

Toxin (Protein)


Gene name higB Uniprot ID A0A8B3FNM7
Locus tag GMW39_RS16820 Protein ID WP_033070820.1
Coordinates 3595637..3595942 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag GMW39_RS16815 Protein ID WP_161546182.1
Coordinates 3595224..3595640 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GMW39_RS16795 3590515..3590961 - 447 WP_012822200.1 helix-turn-helix transcriptional regulator -
GMW39_RS16800 3591116..3592387 + 1272 WP_121295535.1 MFS transporter -
GMW39_RS16805 3592418..3593770 + 1353 WP_014698586.1 aspartate aminotransferase family protein -
GMW39_RS16810 3593851..3594309 - 459 WP_012822197.1 hypothetical protein -
GMW39_RS16815 3595224..3595640 - 417 WP_161546182.1 helix-turn-helix domain-containing protein Antitoxin
GMW39_RS16820 3595637..3595942 - 306 WP_033070820.1 type II toxin-antitoxin system HigB family toxin Toxin
GMW39_RS16825 3596094..3596354 + 261 WP_012822193.1 type II toxin-antitoxin system RelE/ParE family toxin -
GMW39_RS16830 3596428..3596799 + 372 WP_012822192.1 helix-turn-helix domain-containing protein -
GMW39_RS16835 3598398..3598691 - 294 WP_005973730.1 type I-E CRISPR-associated endoribonuclease Cas2 -
GMW39_RS16840 3598692..3599609 - 918 WP_012822191.1 type I-E CRISPR-associated endonuclease Cas1 -
GMW39_RS16845 3599637..3600284 - 648 WP_012822190.1 type I-E CRISPR-associated protein Cas6/Cse3/CasE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(18-90)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-136)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 12010.78 Da        Isoelectric Point: 10.0386

>T144106 WP_033070820.1 NZ_CP046376:c3595942-3595637 [Pectobacterium parmentieri]
VFSRAPFDTAIAQFPNQAAALADLYRTIKREIYATPDDMKKRFPSLDRMKYREKWWVIDIGGGHLRVMFFADFERGKIFI
KHITSHAEYGSLTEYYRRNKE

Download         Length: 306 bp

>T144106 NZ_CP061744:c2735487-2735384 [Enterobacter hormaechei]
GGCAAGGCGATTGAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15330.69 Da        Isoelectric Point: 4.4634

>AT144106 WP_161546182.1 NZ_CP046376:c3595640-3595224 [Pectobacterium parmentieri]
MMYADAIKAANNLTSIVPLLGGSTSCKDYEDALKLIEYLLESEPDSPLIDMLTAKIDKYEEDAPEFVAFNERIKALPAGI
ALLRILMDQHGLTQSDFEAEIGKKSLVSRILNGERTLTLDHMRALAKRFDIPVSAFID

Download         Length: 417 bp

>AT144106 NZ_CP061744:2735381-2735580 [Enterobacter hormaechei]
GATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGG
CATTAATGCAGGCTCAATCGCCTTGCCCTTTAAGAATAGATGACGACGTCAGGTTTTCCAGTCCACAGTAAAAGTGGTCT
GAAAAAAAGCGTCAGAACATCACTAAATGTGAAAAACCGC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B3FNM7

Antitoxin

Source ID Structure

References