Detailed information of TA system TA144098

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 214032..214630
• Replicon: chromosome
• Accession: NZ_CP046376
• Organism: Pectobacterium parmentieri strain HC

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I1YFL9
• Locus tag: GMW39_RS01050
• Protein ID: WP_001896384.1
• Coordinates: 214307..214630 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GMW39_RS01045
• Protein ID: WP_000058216.1
• Coordinates: 214032..214310 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GMW39_RS01030	210993..212813	+	1821	WP_161545416.1	conjugative transfer system coupling protein TraD	-
GMW39_RS01035	212823..213383	+	561	WP_145573973.1	conjugative transfer protein	-
GMW39_RS01040	213370..214006	+	637	Protein_202	DUF4400 domain-containing protein	-
GMW39_RS01045	214032..214310	-	279	WP_000058216.1	helix-turn-helix domain-containing protein	Antitoxin
GMW39_RS01050	214307..214630	-	324	WP_001896384.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GMW39_RS01055	214811..215092	+	282	WP_000433889.1	type IV conjugative transfer system protein TraL	-
GMW39_RS01060	215089..215715	+	627	WP_000667170.1	hypothetical protein	-
GMW39_RS01065	215699..216595	+	897	WP_161546444.1	type-F conjugative transfer system secretin TraK	-
GMW39_RS01070	216598..217887	+	1290	WP_161545417.1	TraB/VirB10 family protein	-
GMW39_RS01075	217962..218534	+	573	WP_161545418.1	type IV conjugative transfer system lipoprotein TraV	-
GMW39_RS01080	218531..218917	+	387	WP_161545419.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	ant(3'')-Ia	-	160473..274251	113778

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12281.33 Da
• Isoelectric Point: 9.7658

>T144098 WP_001896384.1 NZ_CP046376:c214630-214307 [Pectobacterium parmentieri]
MSWKIDFYDGVEDQILDMPPKIQARMIKLLELMEKHGANLGPPHTESMGDGLFEIRAKAQEGIGRGLFCYLKGKHIYVLH
AFVKKSQKTPKNELNLARDRQKEVQKS

Nucleotide

• Length: 324 bp

>T144098 NZ_CP061723:4948551-4948844 [Pseudomonas putida]
ATGACTGAAACCTTCTCTGAATTCGATCCTGCCGCGTACCTCACTACACCTGAAGCAATCGCGCAGTTCATGACCGATGC
ACTTGAAACCGGCGATGCCAGCTATGTCGCTAAAGCTGTCGGCGTGGTTGCACGTGCCAAGGGCATGAGCGAACTGGCGA
AAGAGACGGGGCTGTCCCGAGAGCAGCTTTACAGGTCATTCAGCGATCATGGCAACCCCACGCTGAAGTCGTTTCTGACC
GTGATGAAGGCATTGGGAATAGACATGACGGCGCGGCCTCACCTCGCTGGCTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10517.07 Da
• Isoelectric Point: 9.9393

>AT144098 WP_000058216.1 NZ_CP046376:c214310-214032 [Pectobacterium parmentieri]
MSLQKLKQRALANAEVKNEYDKLESEFSFIDQLLTMRTKAGLTQEQVAERMHTQKSNVSRLEKGNANPSWSTLLKYAHAC
GFELTLKPQKMS

Nucleotide

• Length: 279 bp

>AT144098 NZ_CP061723:4948256-4948558 [Pseudomonas putida]
ATGAAGAAAATCGAATCCAGCAGTTTCAGGCATTGGGTGACCGGACTACGGGACTTAAGCGCCAGAGCCCGAATCATTTC
TCGGATCAACCGGTTGATGGAGGGTCTGCCGGGCGACGTATCTCCGGTAGGCCATGGCGTGAGTGAGCTCAAAATTCACT
ATGGTCCCGGCTATCGGGTGTACTTCCATCAGACGGGCAGCACCTTCGTCATTCTGCTCTGTGGCGGCGACAAAAGCAGT
CAAAAGCGAGACATCAAGGTTGCCCACCAGATTCTGCGTTCGTGGAGGATGCAAAATGACTGA

Structures

Toxin

• AlphaFold DB: A0A244CN87