Detailed information of TA system TA144026

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-couple_hipB
• Location: 1419235..1419883
• Replicon: chromosome
• Accession: NZ_CP046335
• Organism: Streptococcus mitis strain FDAARGOS_684

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V8IC77
• Locus tag: FOB92_RS06860
• Protein ID: WP_000556942.1
• Coordinates: 1419518..1419883 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: V8I9N0
• Locus tag: FOB92_RS06855
• Protein ID: WP_000797746.1
• Coordinates: 1419235..1419528 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOB92_RS06845	1415491..1416357	-	867	WP_000089956.1	methylenetetrahydrofolate reductase [NAD(P)H]	-
FOB92_RS06850	1416461..1418710	-	2250	WP_000108228.1	5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase	-
FOB92_RS06855	1419235..1419528	-	294	WP_000797746.1	helix-turn-helix transcriptional regulator	Antitoxin
FOB92_RS06860	1419518..1419883	-	366	WP_000556942.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FOB92_RS06865	1420039..1420404	-	366	WP_001241814.1	TIGR02328 family protein	-
FOB92_RS06870	1420388..1420792	-	405	WP_000527884.1	YbgA family protein	-
FOB92_RS06875	1420860..1421561	-	702	WP_000748126.1	hypothetical protein	-
FOB92_RS06880	1421696..1423084	-	1389	WP_115262365.1	hypothetical protein	-
FOB92_RS06885	1423081..1424148	-	1068	WP_000415506.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (36-110)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-88)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 14545.72 Da
• Isoelectric Point: 9.3143

>T144026 WP_000556942.1 NZ_CP046335:c1419883-1419518 [Streptococcus mitis]
VHTIYFYKDKNGNEPVLDYMRELACRKSKDSRIKLNKLNDYIELLSQQGTRAGEPYMKHLEDEIWELRPLRDRILFVAWL
DGSFVLLHHFVKKTQKTPRREIEKAKRELKDLKERGVSDEE

Nucleotide

• Length: 366 bp

>T144026 NZ_CP061640:c3659185-3658796 [Acinetobacter seifertii]
TTGATCAACCAATTGAATTTTAAACTGAAATATAGCCGTTTCGCGATTGTATTTCAGTTTTTTATTGGCCTATGTTTAGC
AGCTCTGTTTTATCAGCTGATACCAATACTCTGGTGGTTGGTTACGCTCTGTTTATTATTTATAAGTTTTATTTTCTTTT
TAAAGCGACCACAGCTCGTTCAAATTGCCTATTTAGATCAGAAATTATGGTCTCTTAGCTTTCATTCAGAACAAACCATT
TCTCGTGTAGAAATTTTAAAAATAATTGATTATCAGATTTTCATAGTGGTCTATTTTAAGGGTGATAAAACATTAACTTC
TGTTATTTGGTTTGATCAAATGTCCTTATCTCAATGGAAACAGCTTAAGACTTTAGAAAAATTCTATTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10765.46 Da
• Isoelectric Point: 7.0172

>AT144026 WP_000797746.1 NZ_CP046335:c1419528-1419235 [Streptococcus mitis]
MKNSAIGSNWKDVRSELFTKEEILESDMRVAIMSELIEARHEQGISQKKLEELSGVSQPVIARMETGKTSPQLDTVLKVL
ASLGKTLAVVPLEQRKS

Nucleotide

• Length: 294 bp

>AT144026 NZ_CP061640:c3659437-3659192 [Acinetobacter seifertii]
ATGACCTTGGAAGAACGTAAAGTAGTTTATCGCGCACGCCGCGGTTTAAAGGAAATTGATGTCTATTTTGACCCGTATGT
GAAAAATTATTATTTAAAGGCAGATCCAGCAGAAAAAGCATTGTTTGCTGAATTAGTAGAGCAGGAAGATCCTGATTTAC
TTGACTGGTTTATGGAAGTATCTGAACCGCCGCGTACAGAATTACGTGATTTTATTCATAAATTAAAGCATTATGTTCAT
GGCTGA

Structures

Toxin

• AlphaFold DB: V8IC77

Antitoxin

• AlphaFold DB: V8I9N0