Detailed information of TA system TA144022

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 193777..194418
• Replicon: plasmid unnamed3
• Accession: NZ_CP046333
• Organism: Cupriavidus metallidurans strain FDAARGOS_675

Toxin (Protein)

• Gene name: graT
• Uniprot ID: Q1LE01
• Locus tag: FOB83_RS21260
• Protein ID: WP_011519196.1
• Coordinates: 193777..194061 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FOB83_RS21265
• Protein ID: WP_011519197.1
• Coordinates: 194071..194418 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOB83_RS21230	188793..189194	+	402	WP_011519190.1	hypothetical protein	-
FOB83_RS21235	189277..189867	-	591	WP_011519191.1	hypothetical protein	-
FOB83_RS21240	189990..190676	-	687	WP_011519192.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
FOB83_RS21245	191029..191919	-	891	WP_011519194.1	helix-turn-helix transcriptional regulator	-
FOB83_RS21250	192229..192447	+	219	WP_008651241.1	YdcH family protein	-
FOB83_RS21255	192449..193600	+	1152	WP_011519195.1	polyphosphate kinase 2	-
FOB83_RS21260	193777..194061	+	285	WP_011519196.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FOB83_RS21265	194071..194418	+	348	WP_011519197.1	HigA family addiction module antidote protein	Antitoxin
FOB83_RS21270	194533..195846	+	1314	WP_011519198.1	hemolysin family protein	-
FOB83_RS21275	196246..197583	+	1338	WP_011519199.1	EAL domain-containing protein	-
FOB83_RS21280	197728..197856	+	129	WP_008651235.1	entericidin A/B family lipoprotein	-
FOB83_RS21285	197975..198616	-	642	WP_011519200.1	bifunctional 4-hydroxy-2-oxoglutarate aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	plc / adeG / acrB / flgG / flgC / flhA / cheY / cheB / cheW / motA / kdsA / katA / katA / fliP / fliI	1..2586495	2586495

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (23-99)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10505.15 Da
• Isoelectric Point: 8.5384

>T144022 WP_011519196.1 NZ_CP046333:193777-194061 [Cupriavidus metallidurans]
MIRSLSDKTTAAIYCGQSVHTLPAHIQADALRKLAILDAASSLPDMVAPPANRLEPVLGKRRGHWRVRIKGPWHLCFRFI
NGEAWDVGIAEYEE

Nucleotide

• Length: 285 bp

>T144022 NZ_CP061632:c28266-27907 [Acinetobacter seifertii]
ATGGCTTGGGATGTAGAAACAACAGAACTTTTTGATTCTTGGTTGGCAGAACAAGATGAAAATGCTCAAGATAAAATCTT
AGCGTCTTTGCTTGTTTTGTCAGAACTGGGGCCAAATCTAGGCCGGCCTCATGTTGATACTATTAAAGAATCAAAATATC
CGAACATGAAAGAGATACGTGTGCAAGTTAAAGGTCATCCGATCCGTGGTTTCTTTGCATTTGATCCAGAACGTAAAGCC
ATTGTTTTGTGTGCTGGAGATAAGAAAGGGCTAAATGAAAAAGCCTTTTATAAAGAAATGATCAAGATTGCAGATGAACA
ATATGAGCAATATTTACGTGATAATTACGGAGATAAATAA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 12691.46 Da
• Isoelectric Point: 7.3198

>AT144022 WP_011519197.1 NZ_CP046333:194071-194418 [Cupriavidus metallidurans]
MTINREDLRRADFRSIATGQPIPPAHPGDVLETEFMAPNELSAEALAVALHMAPQRIHDIVRRQRAISPDTALRLARYFG
TSAEFWAGLQTAYALRLARATAGANIERDIQPLTA

Nucleotide

• Length: 348 bp

>AT144022 NZ_CP061632:c27907-27608 [Acinetobacter seifertii]
ATGGCTAAATTAAACGATATAATTTCAAAACGCTCACCTGAAAGCCAACAACGCATCAAGAAAATGGCAAATCAAATGCT
ATTGGATATTAACCTATCGAACCTACGGGAAGAGTTAAAAATATCTCAAGCTGAAATTGCAAAAAATCTAGGTGTAAGCC
AAGCTGCTATTTCAAAACGTGAAAAATTAGGTAAAGATCTTAAGATTTCATCATTAAAAGAATATGTTGAAGCTATGGGT
GGCAAAGTAAGGTTAGATGTTGAGTTACCTAACGGAAAGCATGTTGCTATATCTATTTAA

Structures

Toxin

• AlphaFold DB: A0A132H834