Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2227781..2228451 Replicon chromosome
Accession NZ_CP046308
Organism Mycobacterium tuberculosis strain FDAARGOS_751

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag FOC59_RS10535 Protein ID WP_003899954.1
Coordinates 2227781..2228125 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag FOC59_RS10540 Protein ID WP_003899955.1
Coordinates 2228122..2228451 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOC59_RS10500 2222854..2223714 + 861 WP_003416628.1 alpha/beta hydrolase -
FOC59_RS10505 2223689..2224204 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
FOC59_RS10515 2224444..2224737 + 294 WP_003416635.1 hypothetical protein -
FOC59_RS10520 2225025..2226314 + 1290 WP_003416640.1 ATP-binding protein -
FOC59_RS10525 2226661..2227095 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
FOC59_RS10530 2227098..2227550 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
FOC59_RS10535 2227781..2228125 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FOC59_RS10540 2228122..2228451 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
FOC59_RS10550 2230347..2230694 + 348 WP_003899956.1 DUF2384 domain-containing protein -
FOC59_RS10555 2230691..2231311 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
FOC59_RS10560 2231471..2232736 - 1266 Protein_2069 hypothetical protein -
FOC59_RS10565 2232904..2233113 + 210 WP_003416778.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T143912 WP_003899954.1 NZ_CP046308:2227781-2228125 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T143912 NZ_CP061406:75557-75889 [Klebsiella pneumoniae]
ATGGTATCTCGTTTTGTGCCGGATGCTGGCGACCTTATCTGGATAAATTTTGATCCCGTGGAAGGACATGAGCAAGGAGG
GCATCGCCCCGCAGTCGTGCTGAGTCCGTTTGCATACAACAACAAAACAGGTTTGTTGCTGTGCGTACCTTGCACAACAA
AAGTAAAAGGCTATCCCTTTGAAGTTGAATTGCCAGGAGAACGCGATGGTGTAGCTCTTGCAGATCAAATAACGTGTGTA
GACTGGCGAGCCAGAAAAGTAGAAAAGAAGGGAAAGGTTGCCACCGGAGAATTGTCAGAAATCAGAGCTAAAGCAAAAGC
CTTAATCGGTTGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT143912 WP_003899955.1 NZ_CP046308:2228122-2228451 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT143912 NZ_CP061406:75312-75557 [Klebsiella pneumoniae]
ATGTCACAGGCAACGTTCAAGAAATGGGGTAACCAGGCAGGAATTCGTTTTCCGGCAGCGGTGATGAAAGCAGCAGCGCT
TAACGTAGATGATACGGTTGATATTGAGGTACAGGAAGGGCGGATTGTACTCATACCAACGAAAGAAAAGACCTATTCAC
TCGATGCCTTATTATCCGGCATCACCGAAGAAAACATGCATAACAAAGCTGACTTCGGCAAACCGACAGGGAAGGAAATG
CTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References