Detailed information of TA system TA143894

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 938823..939742
• Replicon: chromosome
• Accession: NZ_CP046308
• Organism: Mycobacterium tuberculosis strain FDAARGOS_751

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: L7N4R2
• Locus tag: FOC59_RS04345
• Protein ID: WP_003900449.1
• Coordinates: 939137..939742 (-)
• Length: 202 a.a.

Antitoxin (Protein)

• Gene name: HigA2
• Uniprot ID: L7N5K9
• Locus tag: FOC59_RS04340
• Protein ID: WP_003410124.1
• Coordinates: 938823..939128 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOC59_RS04310	934206..935090	-	885	Protein_850	13E12 repeat family protein	-
FOC59_RS04315	935444..936019	+	576	WP_003410100.1	hypothetical protein	-
FOC59_RS04320	936016..937056	+	1041	WP_003410103.1	ImmA/IrrE family metallo-endopeptidase	-
FOC59_RS04325	937298..938017	+	720	WP_003410108.1	DUF433 domain-containing protein	-
FOC59_RS04330	938007..938423	+	417	WP_003410114.1	hypothetical protein	-
FOC59_RS04335	938439..938738	-	300	WP_003410120.1	hypothetical protein	-
FOC59_RS04340	938823..939128	-	306	WP_003410124.1	XRE family transcriptional regulator	Antitoxin
FOC59_RS04345	939137..939742	-	606	WP_003900449.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FOC59_RS04350	939767..940126	-	360	WP_003410131.1	hypothetical protein	-
FOC59_RS04355	940286..940963	-	678	WP_003900450.1	hypothetical protein	-
FOC59_RS04360	940915..941151	-	237	WP_003410133.1	hypothetical protein	-
FOC59_RS04365	941358..942254	-	897	WP_003410136.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (86-181)

Antitoxin

• HigA2-like, helix-turn-helix domain (32-92)

Sequences

Toxin

Protein

• Length: 202 a.a.
• Molecular weight: 22744.96 Da
• Isoelectric Point: 7.3073

>T143894 WP_003900449.1 NZ_CP046308:c939742-939137 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Nucleotide

• Length: 606 bp

>T143894 NZ_CP061399:c4822013-4821480 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11291.91 Da
• Isoelectric Point: 10.7482

>AT143894 WP_003410124.1 NZ_CP046308:c939128-938823 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Nucleotide

• Length: 306 bp

>AT143894 NZ_CP061399:c4822290-4822024 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• AlphaFold DB: A0A7U4BV20

Antitoxin

• AlphaFold DB: A0A7U4BV30