Detailed information of TA system TA143784

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 24864..25133
• Replicon: plasmid p2947-NDM5
• Accession: NZ_CP046261
• Organism: Escherichia coli strain ECO2947

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: GK046_RS24440
• Protein ID: WP_001312861.1
• Coordinates: 24975..25133 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	24864..24929 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GK046_RS24410	20574..21101	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
GK046_RS24415	21159..21392	+	234	WP_000006003.1	DUF905 family protein	-
GK046_RS24420	21453..23476	+	2024	Protein_31	ParB/RepB/Spo0J family partition protein	-
GK046_RS24425	23545..23979	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
GK046_RS24430	23976..24695	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	24707..24931	+	225	NuclAT_0	-	-
-	24707..24931	+	225	NuclAT_0	-	-
-	24707..24931	+	225	NuclAT_0	-	-
-	24707..24931	+	225	NuclAT_0	-	-
GK046_RS24435	24716..24895	-	180	WP_001309233.1	hypothetical protein	-
-	24864..24929	+	66	-	-	Antitoxin
GK046_RS24440	24975..25133	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
GK046_RS24845	25371..25748	-	378	Protein_36	hypothetical protein	-
GK046_RS24450	26048..26344	+	297	WP_001272251.1	hypothetical protein	-
GK046_RS24455	26455..27276	+	822	WP_001234445.1	DUF945 domain-containing protein	-
GK046_RS24460	27573..28175	-	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
GK046_RS24465	28498..28881	+	384	WP_001354030.1	relaxosome protein TraM	-
GK046_RS24470	29075..29746	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
GK046_RS24475	29883..30110	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaNDM-5	-	1..66053	66053

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T143784 WP_001312861.1 NZ_CP046261:24975-25133 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T143784 NZ_CP061367:c2828672-2828565 [Shigella sonnei]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT143784 NZ_CP046261:24864-24929 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin