Detailed information of TA system TA143780

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 59416..59685
• Replicon: plasmid p2974-D
• Accession: NZ_CP046260
• Organism: Escherichia coli strain ECO2947

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: GK046_RS23995
• Protein ID: WP_001312861.1
• Coordinates: 59527..59685 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	59416..59481 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GK046_RS23965	55272..55724	+	453	WP_000290842.1	single-stranded DNA-binding protein	-
GK046_RS23970	55786..56019	+	234	WP_000006030.1	DUF905 family protein	-
GK046_RS23975	56084..58042	+	1959	WP_063072993.1	ParB/RepB/Spo0J family partition protein	-
GK046_RS23980	58097..58531	+	435	WP_000845907.1	conjugation system SOS inhibitor PsiB	-
GK046_RS23985	58528..59247	+	720	WP_001276240.1	plasmid SOS inhibition protein A	-
GK046_RS23990	59259..59447	-	189	WP_001299721.1	hypothetical protein	-
-	59259..59483	+	225	NuclAT_0	-	-
-	59259..59483	+	225	NuclAT_0	-	-
-	59259..59483	+	225	NuclAT_0	-	-
-	59259..59483	+	225	NuclAT_0	-	-
-	59416..59481	+	66	-	-	Antitoxin
GK046_RS23995	59527..59685	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
GK046_RS24820	60376..60582	+	207	WP_033804333.1	hypothetical protein	-
GK046_RS24010	60607..60894	+	288	WP_000107535.1	hypothetical protein	-
GK046_RS24015	61014..61835	+	822	WP_001241365.1	DUF945 domain-containing protein	-
GK046_RS24020	62132..62734	-	603	WP_000243705.1	transglycosylase SLT domain-containing protein	-
GK046_RS24025	63055..63438	+	384	WP_001151524.1	relaxosome protein TraM	-
GK046_RS24030	63625..64314	+	690	WP_000283379.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / qnrS1 / aph(6)-Id / aph(3'')-Ib / sul2	stcE / exeE / exeG	1..108424	108424

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T143780 WP_001312861.1 NZ_CP046260:59527-59685 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T143780 NZ_CP046260:59527-59685 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT143780 NZ_CP046260:59416-59481 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin