Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2054524..2055323 Replicon chromosome
Accession NZ_CP046259
Organism Escherichia coli strain ECO2947

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag GK046_RS09940 Protein ID WP_000347267.1
Coordinates 2054859..2055323 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag GK046_RS09935 Protein ID WP_001307405.1
Coordinates 2054524..2054859 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GK046_RS09920 2050309..2051079 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
GK046_RS09925 2051095..2052429 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
GK046_RS09930 2052804..2054375 + 1572 WP_001273741.1 galactarate dehydratase -
GK046_RS09935 2054524..2054859 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
GK046_RS09940 2054859..2055323 + 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
GK046_RS09945 2055378..2056187 - 810 WP_000072193.1 aga operon transcriptional regulator AgaR -
GK046_RS09950 2056436..2057716 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
GK046_RS09955 2057739..2058212 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
GK046_RS09960 2058223..2059002 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
GK046_RS09965 2058992..2059870 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
GK046_RS09970 2059888..2060322 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2045376..2055323 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T143766 WP_000347267.1 NZ_CP046259:2054859-2055323 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T143766 NZ_CP061350:971782-972315 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT143766 WP_001307405.1 NZ_CP046259:2054524-2054859 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT143766 NZ_CP061350:971505-971771 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References