Detailed information of TA system TA143714

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 8165363..8165963
• Replicon: chromosome
• Accession: NZ_CP046173
• Organism: Nocardia terpenica strain AUSMDU00012715

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: F6W96_RS36745
• Protein ID: WP_167490420.1
• Coordinates: 8165685..8165963 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: F6W96_RS36740
• Protein ID: WP_167490419.1
• Coordinates: 8165363..8165671 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F6W96_RS36710	8160813..8161718	+	906	WP_167490415.1	LysR family transcriptional regulator	-
F6W96_RS36715	8161861..8162421	+	561	WP_167490416.1	hypothetical protein	-
F6W96_RS36720	8162488..8163270	-	783	WP_167490417.1	hypothetical protein	-
F6W96_RS36725	8163382..8163855	-	474	WP_175041437.1	NUDIX domain-containing protein	-
F6W96_RS36730	8163839..8164522	-	684	WP_203217845.1	hypothetical protein	-
F6W96_RS36735	8164729..8164893	+	165	WP_167490418.1	hypothetical protein	-
F6W96_RS36740	8165363..8165671	-	309	WP_167490419.1	HigA family addiction module antidote protein	Antitoxin
F6W96_RS36745	8165685..8165963	-	279	WP_167490420.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F6W96_RS36750	8166120..8167103	-	984	WP_167492049.1	c-type cytochrome biogenesis protein CcsB	-
F6W96_RS36755	8167106..8168749	-	1644	WP_167490421.1	cytochrome c biogenesis protein ResB	-
F6W96_RS36760	8168749..8169582	-	834	WP_167490422.1	cytochrome c biogenesis CcdA family protein	-
F6W96_RS36765	8169579..8170175	-	597	WP_167490423.1	TlpA family protein disulfide reductase	-
F6W96_RS36770	8170172..8170777	-	606	WP_167492050.1	histidine phosphatase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (19-70)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10665.24 Da
• Isoelectric Point: 9.7892

>T143714 WP_167490420.1 NZ_CP046173:c8165963-8165685 [Nocardia terpenica]
VIQSFKDKEAQKLWTERSAPKLGNVQKVALRKLTMLDAAAQLDDLRVPPANRLEKLKGDREGQHSIRINDQWRICFVWET
AGPRDVEIINYH

Nucleotide

• Length: 279 bp

>T143714 NZ_CP061337:c1957163-1957056 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11519.36 Da
• Isoelectric Point: 9.9105

>AT143714 WP_167490419.1 NZ_CP046173:c8165671-8165363 [Nocardia terpenica]
MANATPLPPTSPGEILAEEFMEPLRISQNALARALGVPARRINQIVHGKRAITTRTALLLARYFGTTPEFWINLQTHYDL
VREREELSDKLGRIVPRQPLTC

Nucleotide

• Length: 309 bp

>AT143714 NZ_CP061337:1957211-1957277 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTATACCTCTCAACGTGCGGGGGTTTTCTC