Detailed information of TA system TA143700

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 66295..66987
• Replicon: plasmid pTHAF191c_d
• Accession: NZ_CP046165
• Organism: Vibrio sp. THAF191c

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GGC03_RS26885
• Protein ID: WP_152473071.1
• Coordinates: 66295..66579 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GGC03_RS26890
• Protein ID: WP_152473070.1
• Coordinates: 66652..66987 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GGC03_RS26870	64212..65024	-	813	WP_152473073.1	hypothetical protein	-
GGC03_RS26875	65024..65539	-	516	WP_152473072.1	hypothetical protein	-
GGC03_RS26880	65540..65782	-	243	WP_152473095.1	hypothetical protein	-
GGC03_RS26885	66295..66579	+	285	WP_152473071.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GGC03_RS26890	66652..66987	+	336	WP_152473070.1	HigA family addiction module antidote protein	Antitoxin
GGC03_RS26895	67055..67555	-	501	WP_152473069.1	baseplate protein	-
GGC03_RS26900	67572..68192	-	621	WP_152473068.1	hypothetical protein	-
GGC03_RS26905	68202..68882	-	681	WP_152473067.1	hypothetical protein	-
GGC03_RS26910	68903..70483	-	1581	WP_152473066.1	hypothetical protein	-
GGC03_RS26915	70470..70898	-	429	WP_152473065.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..95651	95651

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-91)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (5-84)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11262.71 Da
• Isoelectric Point: 5.6133

>T143700 WP_152473071.1 NZ_CP046165:66295-66579 [Vibrio sp. THAF191c]
MEFRDSYLEDFYENDVAHRKIPSNLENVLFRKLQMLDAATDYRDLLIPPNNQFEHLNGKLAGYSCIRVNRQYRLIFKFVD
GEVVDTYLDPHSYR

Nucleotide

• Length: 285 bp

>T143700 NZ_CP061329:c4628474-4628163 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 11976.87 Da
• Isoelectric Point: 9.7284

>AT143700 WP_152473070.1 NZ_CP046165:66652-66987 [Vibrio sp. THAF191c]
MRNTKRKPVTVGEMLKLEFLEPLAISNSDLASAIGVHRNTISNLINGKTALTSDLANKLAAALGNTPEFWLNIQHAVDVW
DSKNTFEVAKKAITPLVKIESSMLAGGYALA

Nucleotide

• Length: 336 bp

>AT143700 NZ_CP061329:c4628162-4627872 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG