Detailed information of TA system TA143694

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 32358..32961
• Replicon: plasmid unnamed2
• Accession: NZ_CP046157
• Organism: Leptolyngbya sp. BL0902

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GFS31_RS20010
• Protein ID: WP_198808550.1
• Coordinates: 32358..32642 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GFS31_RS20015
• Protein ID: WP_198808537.1
• Coordinates: 32653..32961 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GFS31_RS19995	27536..28228	-	693	WP_198808534.1	PAS domain-containing protein	-
GFS31_RS20000	28828..31776	-	2949	WP_198808535.1	PAS domain-containing protein	-
GFS31_RS20005	32094..32300	-	207	WP_198808536.1	hypothetical protein	-
GFS31_RS20010	32358..32642	+	285	WP_198808550.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GFS31_RS20015	32653..32961	+	309	WP_198808537.1	HigA family addiction module antidote protein	Antitoxin
GFS31_RS20020	33060..33218	-	159	WP_198808538.1	hypothetical protein	-
GFS31_RS20025	33231..33497	-	267	WP_198808539.1	hypothetical protein	-
GFS31_RS20030	33487..37881	-	4395	WP_198808540.1	diguanylate cyclase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..93510	93510

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-94)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-68)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10947.53 Da
• Isoelectric Point: 8.9820

>T143694 WP_198808550.1 NZ_CP046157:32358-32642 [Leptolyngbya sp. BL0902]
VIIGFKSDETKRIFEGFQSRQYLPEIQKIALRKLLLIHAAISVNDLRIPPGNRLEKLAGDRQGQYSIRINNQWRICFAWT
DGNHADQVEIVDYH

Nucleotide

• Length: 285 bp

>T143694 NZ_CP061329:2722676-2722783 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11813.39 Da
• Isoelectric Point: 6.2217

>AT143694 WP_198808537.1 NZ_CP046157:32653-32961 [Leptolyngbya sp. BL0902]
MNSERLPNIHPGEILRLEFLDPLSITPYRLSKDIGVSQTRISEILSEKRSITADTALRLSKYFGNSAQFWMNLQAQYDLR
QAQEENRDIYEHIPVAQLAHLT

Nucleotide

• Length: 309 bp

>AT143694 NZ_CP061329:c2722629-2722563 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT