Detailed information of TA system TA143689

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1002755..1003345
• Replicon: chromosome
• Accession: NZ_CP046155
• Organism: Leptolyngbya sp. BL0902

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: GFS31_RS04535
• Protein ID: WP_198807071.1
• Coordinates: 1003064..1003345 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GFS31_RS04530
• Protein ID: WP_198807070.1
• Coordinates: 1002755..1003054 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GFS31_RS04520	999709..1001136	+	1428	WP_198808032.1	pyruvate kinase	-
GFS31_RS04525	1001272..1002648	+	1377	WP_198807069.1	FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO	-
GFS31_RS04530	1002755..1003054	-	300	WP_198807070.1	HigA family addiction module antidote protein	Antitoxin
GFS31_RS04535	1003064..1003345	-	282	WP_198807071.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GFS31_RS04540	1003635..1004294	+	660	WP_198807072.1	esterase	-
GFS31_RS04545	1004398..1004610	+	213	WP_198807073.1	DUF2555 domain-containing protein	-
GFS31_RS04550	1004658..1005917	+	1260	WP_198808033.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-70)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11117.74 Da
• Isoelectric Point: 10.5317

>T143689 WP_198807071.1 NZ_CP046155:c1003345-1003064 [Leptolyngbya sp. BL0902]
MIRSFKDKEAQAIFERRRSRKVPSEIQQVALRKLRMLNRAETLQDLRIPPANRLERLSGDRSGQYSIRINDQWRICFVWQ
AGEAFDVEIVDYH

Nucleotide

• Length: 282 bp

>T143689 NZ_CP061329:1807576-1807827 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11436.07 Da
• Isoelectric Point: 5.1200

>AT143689 WP_198807070.1 NZ_CP046155:c1003054-1002755 [Leptolyngbya sp. BL0902]
MDNERLRPVHPGEILLEEFLQPMNLSQNQLALALRVPPRRINEIVHGNRRITADTALRLAHYFGMSPRFWLGLQMDYDLD
VAEDEIGDRLSREVVALNS

Nucleotide

• Length: 300 bp

>AT143689 NZ_CP061329:1807824-1808078 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA