Detailed information of TA system TA143683

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1183316..1183905
• Replicon: chromosome
• Accession: NZ_CP046148
• Organism: Xanthomonas oryzae pv. oryzae strain ITCCBB0002

Toxin (Protein)

• Gene name: graT
• Uniprot ID: G7TIR7
• Locus tag: GKO49_RS05635
• Protein ID: WP_012443754.1
• Coordinates: 1183624..1183905 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GKO49_RS05630
• Protein ID: WP_011260746.1
• Coordinates: 1183316..1183606 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GKO49_RS05600	1178601..1179077	-	477	WP_011260741.1	DUF4123 domain-containing protein	-
GKO49_RS05605	1179080..1179514	-	435	WP_011260742.1	PAAR domain-containing protein	-
GKO49_RS05610	1179630..1179875	-	246	WP_069960049.1	hypothetical protein	-
GKO49_RS05615	1180211..1180543	+	333	WP_011260743.1	hypothetical protein	-
GKO49_RS05625	1180993..1182387	-	1395	WP_027703652.1	nucleoside hydrolase	-
GKO49_RS05630	1183316..1183606	-	291	WP_011260746.1	HigA family addiction module antidote protein	Antitoxin
GKO49_RS05635	1183624..1183905	-	282	WP_012443754.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GKO49_RS05640	1184000..1186258	-	2259	WP_075240794.1	exodeoxyribonuclease V subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11066.83 Da
• Isoelectric Point: 10.5318

>T143683 WP_012443754.1 NZ_CP046148:c1183905-1183624 [Xanthomonas oryzae pv. oryzae]
VIRSFIDKDAEKIWLGERSRRLPADIQLVARRKLRMLNAAAHLDDLRIPPANRLEALKGQRRGQYSIRINDQWRICFRWM
EGDVVQVEIVDYH

Nucleotide

• Length: 282 bp

>T143683 NZ_CP061321:c285098-284802 [Polynucleobacter sp. MWH-UH2A]
TTGCAATATAATCCTCAGATAAAAGTAATCACTAATCGTCGTTTGCTGGATTTTCCTGAACGTTACAAAGATGCTTTTGA
ACCCCTTCAATCCTGGAGAAAATTGATCGAATTTGGATCCTACAAAAACTTTGCAGAATTAAAGCAAGTTTTCAGATCAG
CTGACAAGGTTGGGGATTTGTTTGTTTTTAATATTTGTGGAAATAAATATCGTTTAATTACATTTTTACAGTTTGAAAGA
CAGCTTTGTTACATCAAAGCTGTTTTAACGCATAAAGAATATGACAAAGATGGGTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10420.07 Da
• Isoelectric Point: 7.2040

>AT143683 WP_011260746.1 NZ_CP046148:c1183606-1183316 [Xanthomonas oryzae pv. oryzae]
MTVLPNIHPGDILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERFWLGLQADYELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT143683 NZ_CP061321:c284796-284386 [Polynucleobacter sp. MWH-UH2A]
ATGAACTTAAATGACCTCAAAAAGGCTTGGGAAAATCTATCTAAAACAGTTAATCTAAGTCCCATTCGCACTGAAAAACA
ATACAAGAAAATGGTGCAATTGGCAGATATGCTTTCGGATGTCATTGCCTCTTCAAAAAAACATTCTTTACTGGATCTAT
TTGAACTAGTAAGCGAGCTCATTCGCGCATACGAACACGAGCATTTTCCAGTTAAAGATGCGAAGCCCCAAGATATCTTG
GCGTTCTTAATGCAAGAACACCATCTAAAGCAATCCGATTTACCTGAAGTTGGAAATCAAAGCGTCATCTCACAAATCCT
AGCAGGAGCCCGTCAACTTAATGTAAGGCAAATTAATGCATTAGCTAAAAGATTTAAGGTGTCAGCTGGAGTATTTTTTG
AGCGAGCCTAA

Structures

Toxin

• AlphaFold DB: A0A0K0GFW5