Detailed information of TA system TA143672

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 73948..74201
• Replicon: plasmid pTEM-CBG
• Accession: NZ_CP046117
• Organism: Enterobacter cloacae strain CBG15936

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: GJ694_RS24360
• Protein ID: WP_001312851.1
• Coordinates: 74052..74201 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	73948..74007 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJ694_RS24340	70675..71421	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
GJ694_RS24345	71476..72036	+	561	WP_000139328.1	fertility inhibition protein FinO	-
GJ694_RS24350	72168..72368	+	201	WP_015059022.1	hypothetical protein	-
GJ694_RS24355	72754..73353	+	600	WP_032083981.1	hypothetical protein	-
-	73948..74007	-	60	NuclAT_1	-	Antitoxin
-	73948..74007	-	60	NuclAT_1	-	Antitoxin
-	73948..74007	-	60	NuclAT_1	-	Antitoxin
-	73948..74007	-	60	NuclAT_1	-	Antitoxin
GJ694_RS24360	74052..74201	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
GJ694_RS24365	74485..74733	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB	-	1..75044	75044

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T143672 WP_001312851.1 NZ_CP046117:74052-74201 [Enterobacter cloacae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T143672 NZ_CP061316:c267356-267066 [Polynucleobacter sp. AP-Jannik-300A-C4]
TTGAAGACCTATAGTCTTGAGTTCAATGTACTTGCTTTAAAAGAGTGGCAAAAATTAGATAACTCAGTTCGCAGCCAGTT
CAAAAAGCAGCTTGAGAAACGAATTCAAAATCCTAGAATTGCTTCAGCCAAGTTACATGGTGACCTAGATAATTTCTACA
AGATAAAACTAGCAGCACTTGGCTACAGGCTAGTTTATGAAGTTATAGACCACAGACTTATTGTTCTGATTATTGCAGTT
GGCAAGCGAAATCAGTCGGCTGTTTATAAAGAAGCAGGCAAAAGAAAATAG

Antitoxin

Nucleotide

• Length: 60 bp

>AT143672 NZ_CP046117:c74007-73948 [Enterobacter cloacae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin