Detailed information of TA system TA143668

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 34487..34757
• Replicon: plasmid pTEM-CBG
• Accession: NZ_CP046117
• Organism: Enterobacter cloacae strain CBG15936

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: GJ694_RS24135
• Protein ID: WP_001312861.1
• Coordinates: 34599..34757 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	34487..34550 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJ694_RS24110	30198..30725	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
GJ694_RS24115	30783..31016	+	234	WP_000006003.1	DUF905 family protein	-
GJ694_RS24120	31077..33100	+	2024	Protein_40	ParB/RepB/Spo0J family partition protein	-
GJ694_RS24125	33169..33603	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
GJ694_RS24130	33600..34319	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	34331..34555	+	225	NuclAT_0	-	-
-	34331..34555	+	225	NuclAT_0	-	-
-	34331..34555	+	225	NuclAT_0	-	-
-	34331..34555	+	225	NuclAT_0	-	-
-	34487..34550	-	64	-	-	Antitoxin
GJ694_RS24135	34599..34757	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
GJ694_RS24820	34995..35372	-	378	Protein_44	hypothetical protein	-
GJ694_RS24145	35672..35968	+	297	WP_001272251.1	hypothetical protein	-
GJ694_RS24150	36079..36900	+	822	WP_001234445.1	DUF945 domain-containing protein	-
GJ694_RS24155	37197..37844	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
GJ694_RS24160	38121..38504	+	384	WP_000124981.1	relaxosome protein TraM	-
GJ694_RS24165	38695..39381	+	687	WP_015059009.1	PAS domain-containing protein	-
GJ694_RS24170	39475..39702	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB	-	1..75044	75044

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T143668 WP_001312861.1 NZ_CP046117:34599-34757 [Enterobacter cloacae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T143668 NZ_CP061315:c1127512-1127231 [Polynucleobacter sp. AP-Kolm-20A-A1]
ATGCGGAGGATTGAGCGTACAACTCAATTCAAAAGGGATTACAAAAGAGAAGCAAAAGGAAGGTATCAAGCTGCCATATC
AACTATTTTGGTAGAGGCCGTGGAATTCCTGGTAATTGATGAGGCATTGCCTGCCAGGTATTTTGATCATGCGCTTACCG
GCCAATGGAGCGATTTTCGGGATTGCCATATCAAGCCAGATCTCATATTGATTTATCAAAAACCCAATTTGGACTTGTTA
CGCTTGGTTAGATTGGGCTCTCATAGCCAGCTAGGCTTGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT143668 NZ_CP046117:c34550-34487 [Enterobacter cloacae]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin