Detailed information of TA system TA143665

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3847712..3848369
• Replicon: chromosome
• Accession: NZ_CP046116
• Organism: Enterobacter cloacae strain CBG15936

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A421IAD7
• Locus tag: GJ694_RS18220
• Protein ID: WP_014832979.1
• Coordinates: 3847712..3848011 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GJ694_RS18225
• Protein ID: WP_038980942.1
• Coordinates: 3848022..3848369 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJ694_RS18205	3843321..3845513	+	2193	WP_129253226.1	type I secretion system permease/ATPase	-
GJ694_RS18210	3845524..3846693	+	1170	WP_096897060.1	HlyD family efflux transporter periplasmic adaptor subunit	-
GJ694_RS24795	3846885..3846999	+	115	Protein_3570	ash family protein	-
GJ694_RS18215	3847021..3847458	+	438	WP_038980939.1	hypothetical protein	-
GJ694_RS18220	3847712..3848011	+	300	WP_014832979.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GJ694_RS18225	3848022..3848369	+	348	WP_038980942.1	HigA family addiction module antidote protein	Antitoxin
GJ694_RS18235	3848516..3849247	-	732	WP_038980944.1	helix-turn-helix transcriptional regulator	-
GJ694_RS18240	3849346..3849765	+	420	WP_058651053.1	nuclear transport factor 2 family protein	-
GJ694_RS18245	3849768..3850286	-	519	WP_029883923.1	hypothetical protein	-
GJ694_RS18250	3850372..3851553	-	1182	WP_038420464.1	PLP-dependent aminotransferase family protein	-
GJ694_RS18255	3851573..3852460	-	888	WP_020690740.1	LysR family transcriptional regulator	-
GJ694_RS18260	3852558..3853160	+	603	WP_013098397.1	short chain dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (25-72)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11646.28 Da
• Isoelectric Point: 10.0182

>T143665 WP_014832979.1 NZ_CP046116:3847712-3848011 [Enterobacter cloacae]
MINHFRDQWLEDFFLYGRSSNVIPSNLETVLARKLDLINAAISHRDLRSPPGNMFEALNPPLKGYSSIRVNRQYRLVFRW
AEGKADDLYLSPHKYTQHK

Nucleotide

• Length: 300 bp

>T143665 NZ_CP061314:c1407705-1407514 [Polynucleobacter corsicus]
ATGCATAGCAAAAAGCTAATTGAGCTTTTAAAGGTTGATGGATGGGTGTTGAGCAGAACAAAAGGATCGCATCACATATT
TACTCATACAACAAAGTCTGGACATGTCTGTATCCCGCATCCAAAAAAAGACTTGGGTTTGGGGTTGGTAGAAAAAATAC
TGAAACAAGCCGGATTAAAGGAGGGGTCTTAG

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13018.00 Da
• Isoelectric Point: 7.0139

>AT143665 WP_038980942.1 NZ_CP046116:3848022-3848369 [Enterobacter cloacae]
MILQQALRKPTTPGDVLQYEYLEPLNLKISDLAEMLNVHRNTISALVNNNRKLTADMAIKLAKAFDTTIEFWLNLQLNVD
IWEAQSNARTQEELSRIKTVAEVIAKRKGGQPDVA

Nucleotide

• Length: 348 bp

>AT143665 NZ_CP061314:c1407513-1407121 [Polynucleobacter corsicus]
ATGAAATATCCAATAGCGATTGAGCCAGGAAACGAGAAAAATGCATGGGGAGTTGTTGTCCCAGATTTGCCTGGTTGTTT
TTCTGCTGCTGACAGTGGTCTTGATGAAGCAATAGATCAGGCCAAAGAGGCTATAGAGCTTTGGATTGAGATGGCTTTAG
ATGGAGGTCAAGCAATACCAAAGCCTAGCTCCTTAAGAGAACTACAAAAGAAAAAAGAATTTAAGGGTTGGGCTTGGGCG
ATTGTGGAAATTGATCCAGCATTGCTTTCGGATGAAATTGAGCGAATCAATATCACCCTGCCAAAAAGAGTATTGGCAAG
ATTAGATGCAAAAGCAAAAAAGGCTGGAGAAAACAGATCTGCATTCATCGCGCATTTGGCCCTAACTGCTTGA

Structures

Toxin

• AlphaFold DB: A0A421IAD7