Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 1218545..1219194 Replicon chromosome
Accession NZ_CP046116
Organism Enterobacter cloacae strain CBG15936

Toxin (Protein)


Gene name higB Uniprot ID A0A5E1A383
Locus tag GJ694_RS05570 Protein ID WP_013097692.1
Coordinates 1218832..1219194 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A5E1A3P3
Locus tag GJ694_RS05565 Protein ID WP_013097693.1
Coordinates 1218545..1218844 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJ694_RS05555 1214950..1216923 + 1974 WP_045902386.1 PhoX family phosphatase -
GJ694_RS05560 1217026..1218414 + 1389 WP_129253495.1 phenylalanine transporter -
GJ694_RS05565 1218545..1218844 - 300 WP_013097693.1 helix-turn-helix transcriptional regulator Antitoxin
GJ694_RS05570 1218832..1219194 - 363 WP_013097692.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GJ694_RS05575 1219400..1220638 + 1239 WP_029882613.1 MFS transporter -
GJ694_RS05580 1220654..1221679 + 1026 WP_038418842.1 sugar phosphate isomerase/epimerase -
GJ694_RS05585 1221672..1222814 + 1143 WP_129253498.1 Gfo/Idh/MocA family oxidoreductase -
GJ694_RS05590 1222890..1223861 + 972 WP_029882610.1 LacI family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13994.00 Da        Isoelectric Point: 7.3235

>T143655 WP_013097692.1 NZ_CP046116:c1219194-1218832 [Enterobacter cloacae]
MWNVETTDRFDEWFFEQTTALKEDVLAAMHILSEFGPQLGRPFVDTVKASAFGNMKELRIQHRGNPIRAFFAFDPDRKAI
VLCAGDKTGCNQKRFYKEMIALADVEYHRHLANKEAIWQR

Download         Length: 363 bp

>T143655 NZ_CP061306:1166248-1166556 [Polynucleobacter sp. JS-JIR-II-b4]
ATGAAAAAAACAGTTACCAGCCCTTATGATGTAGCAGAACATTTGCGCACCCCCAAAGAAATGTCTCTTTATCTACAGGC
GTGTATTGAGGAATCCAACGGAGACGCAGCGTTTATAGCAAAAGCGCTTGGTGACATAGCGCGTGCTAAAGGAATGGCCC
AAGTCGCCAAGGAATCTGGACTCTCAAGAGAAAGCTTATACAAAGCCCTATCTGGTGATCGCATCCCGGGCTTTGATACG
GTATTAAAGGTACTTACAGCCCTTGGCCTTAGCCTGCACACAAAACCAATTAGTAAGATTCGTGCTTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10875.62 Da        Isoelectric Point: 8.6152

>AT143655 WP_013097693.1 NZ_CP046116:c1218844-1218545 [Enterobacter cloacae]
MATLKELMAQQSAESQERIAAKVEVMRRIVALNQLREELNISQTELAAAMGVKQPTVAKIEQPGNDPRLSTLKRYVSALG
GELSIDVTLPNGKRIAFEI

Download         Length: 300 bp

>AT143655 NZ_CP061306:1165949-1166239 [Polynucleobacter sp. JS-JIR-II-b4]
ATGCATGAAATAAGGAAAACAGAAGAATTTAAGTCCTGGTTAGATTCCCTTAATGATTTGATGGGGAGGGCAAAAATTCA
AGCCAGAATTAAACGTTTGGCTGAAGGCAATTCAGGAAATACAAAATCCGTTGGCAGCAAAGTCTTTGAGATGAAGATTG
ATTTTGGACCCGGCTATAGGATTTATTACACAAAACACCAGAGCGCGATATATCTATTACTTATCGGTGGCGATAAAAGA
ACTCAGAGCAAAGATATTCAGATAGCAAAATATTTGGCAAAACACATATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1A383

Antitoxin

Source ID Structure
AlphaFold DB A0A5E1A3P3

References