Detailed information of TA system TA143634

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1225214..1225775
• Replicon: chromosome
• Accession: NZ_CP046114
• Organism: Glaesserella parasuis strain SCW0912

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U4SMA9
• Locus tag: GJV39_RS06405
• Protein ID: WP_010786777.1
• Coordinates: 1225214..1225492 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: GJV39_RS06410
• Protein ID: WP_005712616.1
• Coordinates: 1225503..1225775 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJV39_RS06390	1220996..1222324	-	1329	WP_021109502.1	exodeoxyribonuclease VII large subunit	-
GJV39_RS06395	1222438..1223700	-	1263	WP_021109501.1	serine hydroxymethyltransferase	-
GJV39_RS06400	1224055..1225065	-	1011	WP_021109500.1	ornithine carbamoyltransferase	-
GJV39_RS06405	1225214..1225492	+	279	WP_010786777.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GJV39_RS06410	1225503..1225775	+	273	WP_005712616.1	HigA family addiction module antidote protein	Antitoxin
GJV39_RS06415	1225839..1227059	+	1221	WP_005712618.1	ATP-dependent RNA helicase RhlB	-
GJV39_RS06420	1227069..1227377	+	309	Protein_1232	DUF853 family protein	-
GJV39_RS06425	1227449..1228663	-	1215	WP_021109499.1	ATP-binding protein	-
GJV39_RS06430	1228871..1229389	-	519	WP_012621700.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-81)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10278.66 Da
• Isoelectric Point: 10.0505

>T143634 WP_010786777.1 NZ_CP046114:1225214-1225492 [Glaesserella parasuis]
MIISFKHKGLKLFFETGSTAGIQANHASKLAFQLATLNQAKSALDMNAPSWNLHQLQGNLAGHWSIKVSGNWRLTFKVQN
GHAEVVDYQDYH

Nucleotide

• Length: 279 bp

>T143634 NZ_CP061296:1089908-1090150 [Polynucleobacter sp. MWH-P3-07-1]
ATGGGAAGAGCAAAAATTCAAGCCAGAATTAAACGTTTAGTCGAAGGAAACCCAGGAAATACAAAATCCGTTGGCGGTAA
AGTTTTTGAGATGAAGATTGATTTTGGGCCTGGCTACAGGATTTATTACACAAAATATGAGGATGTGATGTATCTATTAC
TGATCGGAGGCAATAAAAGAACTCAGAGTAAAGATATTCAGATTGCAAAATATTTAGCCAAAAACATACAGGCAATGAGA
TGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10314.06 Da
• Isoelectric Point: 9.8982

>AT143634 WP_005712616.1 NZ_CP046114:1225503-1225775 [Glaesserella parasuis]
MKMYNPAHPGLILREYIEGYNVTEVAKRLGVTRVTLSRILNGKAAISPEMALRLSQLLKTSPELWLGLQSQYDLWQLEQR
PSFNIQPLFA

Nucleotide

• Length: 273 bp

>AT143634 NZ_CP061296:1090147-1090455 [Polynucleobacter sp. MWH-P3-07-1]
ATGAAAAAGACAATTATTAGTCCTTATGATGTAGTGGAGCACTTACGTACCCCAAAGGAAATGGCGCTTTATCTACAAGC
ATGTATTGAAGAATCCGATGGGGATGCGGCGTTTATAGCAAAAGCACTTGGTGATATAGCGCGCGCAAAAGGAATGGCTC
AGGTAGCCAAGGAATCCGGGCTCTCAAGAGAAAGTCTTTATAAAGCTTTGTCTGGCGATCGAATACCGGGGTTTGATACT
GTATTAAAGGTGCTGGCAGCTCTTGGTCTGAGCCTCCATACAAAACCCACTAGTAAAGCTCGTGCTTAA

Structures

Toxin

• AlphaFold DB: U4SMA9