Detailed information of TA system TA143618

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-ratA/-
• Location: 2576017..2576402
• Replicon: chromosome
• Accession: NZ_CP046113
• Organism: Enterococcus faecalis strain 092160007-3

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: A0A4U3MBY2
• Locus tag: GKQ54_RS12085
• Protein ID: WP_002372618.1
• Coordinates: 2576017..2576214 (+)
• Length: 66 a.a.

Antitoxin (RNA)

Gene name	ratA
Locus tag	-
Coordinates	2576147..2576402 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GKQ54_RS12070	2571494..2572126	-	633	WP_002358972.1	RloB family protein	-
GKQ54_RS12075	2572135..2573430	-	1296	WP_002379013.1	AAA family ATPase	-
GKQ54_RS12080	2573892..2575508	+	1617	WP_002379014.1	phosphatase PAP2/LCP family protein	-
GKQ54_RS12085	2576017..2576214	+	198	WP_002372618.1	putative holin-like toxin	Toxin
-	2576147..2576402	-	256	-	-	Antitoxin
GKQ54_RS12090	2576404..2580177	-	3774	WP_025193481.1	WxL domain-containing protein	-
GKQ54_RS12095	2580720..2580917	+	198	WP_002368507.1	putative holin-like toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (32-65)

Sequences

Toxin

Protein

• Length: 66 a.a.
• Molecular weight: 7516.13 Da
• Isoelectric Point: 10.5649

>T143618 WP_002372618.1 NZ_CP046113:2576017-2576214 [Enterococcus faecalis]
MCREVMAVAIQSEREVMRMHVFPKFTERRGLLSAYETIQTILGFGMFTIALIALIVKLLKNDKKK

Nucleotide

• Length: 198 bp

>T143618 NZ_CP061292:c1191420-1191094 [Polynucleobacter sp. MWH-UH24A]
ATGAAGCGAGGCGATATTTATTTGGTATCTCTTGATCCAACAGCTGGCCATGAGCAAAAAGGGTATCGACCTGTATTGGT
CGTGTCACCAGCACCGTTTAATCAACTAACCAAGACCCCAATTGTTGTGCCGATTTCAACTGGCGGTCAGTTTGCCCGGG
TTGCAGGGTTTGCAGTCGAGCTGGTTGGAACTAAAACCACAGGGATTGTTCGCTGCGATCAACCTCGCACCCTAGACCTC
GTGGCGCGTAAAGCAAAACGCGTTGAGACCGTGTCGGCGGCTGTGATTGATGATGTCTTAGCTAAACTGGCAACCTTAGT
CACTTAG

Antitoxin

Nucleotide

• Length: 256 bp

>AT143618 NZ_CP046113:c2576402-2576147 [Enterococcus faecalis]
ATAAAAATGCCCGAGCCTTGAAATAGGAGGCTCAGGCATTTTTTATTGATGGTCTTTTTAGGGTTGTTGTGCTATAATGA
TAAAGAAAAGAGAGATATGCTTCCAACATATCTCTCTGATGTAGAGCCGTTTAAGACGGTGACCAATTTTATTATTTAAA
AATAACCGTGCTTGGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAATTTCACAATCAGCGCAATCAAAGCA
ATGGTAAACATACCAA

Structures

Toxin

• AlphaFold DB: A0A4U3MBY2

Antitoxin