Detailed information of TA system TA143544

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): fst-RNAII/Fst(toxin)
• Location: 51024..51265
• Replicon: plasmid pTEF2
• Accession: NZ_CP046110
• Organism: Enterococcus faecalis strain 133170041-3

Toxin (Protein)

• Gene name: fst
• Uniprot ID: -
• Locus tag: GKQ57_RS14630
• Protein ID: WP_002360667.1
• Coordinates: 51024..51134 (+)
• Length: 37 a.a.

Antitoxin (RNA)

Gene name	RNAII
Locus tag	-
Coordinates	51174..51265 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GKQ57_RS14590	46222..47383	-	1162	Protein_48	IS3 family transposase	-
GKQ57_RS14595	47457..47564	+	108	Protein_49	recombinase family protein	-
GKQ57_RS14600	47554..47868	+	315	WP_002383634.1	hypothetical protein	-
GKQ57_RS14605	47862..48086	+	225	WP_002383635.1	hypothetical protein	-
GKQ57_RS14610	48147..48356	+	210	WP_002382045.1	hypothetical protein	-
GKQ57_RS14615	48368..48670	+	303	WP_002372577.1	hypothetical protein	-
GKQ57_RS14620	49098..50426	+	1329	WP_002400994.1	Y-family DNA polymerase	-
GKQ57_RS14625	50423..50773	+	351	WP_002399364.1	hypothetical protein	-
GKQ57_RS14630	51024..51134	+	111	WP_002360667.1	type I toxin-antitoxin system Fst family toxin	Toxin
-	51174..51265	-	92	NuclAT_0	-	Antitoxin
-	51174..51265	-	92	NuclAT_0	-	Antitoxin
-	51174..51265	-	92	NuclAT_0	-	Antitoxin
-	51174..51265	-	92	NuclAT_0	-	Antitoxin
GKQ57_RS14635	51374..51664	+	291	WP_001137528.1	hypothetical protein	-
GKQ57_RS14640	51768..52139	-	372	WP_000049959.1	replication-associated protein RepC	-
GKQ57_RS14645	52132..52977	-	846	WP_000239313.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..53448	53448
	-	flank	IS/Tn	-	-	46222..47058	836

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 37 a.a.
• Molecular weight: 4117.92 Da
• Isoelectric Point: 4.1672

>T143544 WP_002360667.1 NZ_CP046110:51024-51134 [Enterococcus faecalis]
VLFVKDLMSLVIAPIFVGLVLEMISRVLDEEDDNRK

Nucleotide

• Length: 111 bp

>T143544 NZ_CP061188:c79750-79544 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCTGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 92 bp

>AT143544 NZ_CP046110:c51265-51174 [Enterococcus faecalis]
TAAAAATATGTTATACTAAAGGTGCGAAACGACATTAAATCGTACAAATAACACAAAAAGCAATCCTACGGCGAATAGGA
TTGCTTTTTTTT

Structures

Antitoxin