Detailed information of TA system TA143516

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1994..2599
• Replicon: plasmid pSULFM1
• Accession: NZ_CP046073
• Organism: Sulfurimonas sp. H1576

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GJV85_RS13245
• Protein ID: WP_207563236.1
• Coordinates: 1994..2287 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GJV85_RS13250
• Protein ID: WP_194368098.1
• Coordinates: 2300..2599 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJV85_RS13240	1363..1926	-	564	WP_207563235.1	recombinase family protein	-
GJV85_RS13245	1994..2287	+	294	WP_207563236.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GJV85_RS13250	2300..2599	+	300	WP_194368098.1	HigA family addiction module antidote protein	Antitoxin
GJV85_RS13255	2691..4244	-	1554	WP_207562525.1	IS1182 family transposase	-
GJV85_RS13260	4432..4782	+	351	WP_207563237.1	hypothetical protein	-
GJV85_RS13265	4881..6083	+	1203	WP_207561025.1	IS256 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..81892	81892

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-70)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11139.93 Da
• Isoelectric Point: 8.9327

>T143516 WP_207563236.1 NZ_CP046073:1994-2287 [Sulfurimonas sp. H1576]
MIVSFKDVVTENIFNGVTSKQAMKRLPNKLWKIAARKLDQLDSVIILDELKVPPGNHLEKLSGDRNGQYSIPINQQYRIC
FIWTVSGPDKVEIIDYH

Nucleotide

• Length: 294 bp

>T143516 NZ_CP061176:c1242122-1241376 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTGAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCTGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11707.58 Da
• Isoelectric Point: 7.3184

>AT143516 WP_194368098.1 NZ_CP046073:2300-2599 [Sulfurimonas sp. H1576]
MRIPTHREPTHPGEMLLEEFLEPMNLTQRMLADAIHVPYQRINEIVNMKRGVTPSTALRLAKYFGVSEDFWLNLQTRWDI
YRSKKIEAEELERIKPLAS

Nucleotide

• Length: 300 bp

>AT143516 NZ_CP061176:c1241375-1241205 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA