Detailed information of TA system TA143515

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5619891..5620486
• Replicon: chromosome
• Accession: NZ_CP046069
• Organism: Pseudomonas aeruginosa strain KRP1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: KRP1_RS26220
• Protein ID: WP_003117425.1
• Coordinates: 5620208..5620486 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KRP1_RS26215
• Protein ID: WP_003113527.1
• Coordinates: 5619891..5620196 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KRP1_RS26180	5615030..5615878	+	849	WP_023114865.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
KRP1_RS26190	5616045..5616986	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
KRP1_RS26195	5617103..5617717	+	615	WP_003095013.1	50S ribosomal protein L25/general stress protein Ctc	-
KRP1_RS26200	5617759..5618343	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
KRP1_RS26205	5618384..5619484	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
KRP1_RS26215	5619891..5620196	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
KRP1_RS26220	5620208..5620486	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KRP1_RS26225	5620815..5623043	+	2229	WP_003113525.1	TonB-dependent receptor	-
KRP1_RS26230	5623113..5623760	-	648	WP_003095021.1	carbonate dehydratase	-
KRP1_RS26235	5623822..5625060	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T143515 WP_003117425.1 NZ_CP046069:c5620486-5620208 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T143515 NZ_CP061176:490252-490602 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT143515 WP_003113527.1 NZ_CP046069:c5620196-5619891 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT143515 NZ_CP061176:489965-490246 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: A0A241XLJ5