Detailed information of TA system TA143507

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 13569..14261
• Replicon: plasmid pTHAF191d_d
• Accession: NZ_CP046068
• Organism: Vibrio sp. THAF191d

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GGC04_RS26640
• Protein ID: WP_152473071.1
• Coordinates: 13977..14261 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GGC04_RS26635
• Protein ID: WP_152473070.1
• Coordinates: 13569..13904 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GGC04_RS26610	9658..10086	+	429	WP_152473065.1	hypothetical protein	-
GGC04_RS26615	10073..11653	+	1581	WP_152473066.1	hypothetical protein	-
GGC04_RS26620	11674..12354	+	681	WP_152473067.1	hypothetical protein	-
GGC04_RS26625	12364..12984	+	621	WP_152473068.1	hypothetical protein	-
GGC04_RS26630	13001..13501	+	501	WP_152473069.1	baseplate protein	-
GGC04_RS26635	13569..13904	-	336	WP_152473070.1	HigA family addiction module antidote protein	Antitoxin
GGC04_RS26640	13977..14261	-	285	WP_152473071.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GGC04_RS26645	14774..15016	+	243	WP_152473095.1	hypothetical protein	-
GGC04_RS26650	15017..15532	+	516	WP_152473072.1	hypothetical protein	-
GGC04_RS26655	15532..16344	+	813	WP_152473073.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..95651	95651

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-91)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (5-84)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11262.71 Da
• Isoelectric Point: 5.6133

>T143507 WP_152473071.1 NZ_CP046068:c14261-13977 [Vibrio sp. THAF191d]
MEFRDSYLEDFYENDVAHRKIPSNLENVLFRKLQMLDAATDYRDLLIPPNNQFEHLNGKLAGYSCIRVNRQYRLIFKFVD
GEVVDTYLDPHSYR

Nucleotide

• Length: 285 bp

>T143507 NZ_CP061170:c904345-904094 [Microbacterium sp. HY82]
ATGCCCGCAATCACAGCAACTGAAGCACGCAAGAGCCTGTTCGGACTCATCCAGCGCGTCAACGATGACCACACTGCGGT
CGAGGTTGTTTCCAAGCACGGCAACGCAGTTATCCTCTCGAAAGACGACTACGACGCAATCACCGAGACCGCTTACTTGC
TGCGCAACTCCGCAAACGCTGAGCGTCTACTCGACGCGATCGAGCGCGCTCGACGCGGCGAGTTTGAACAGCACGATCTT
CTCGAAGCATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 11976.87 Da
• Isoelectric Point: 9.7284

>AT143507 WP_152473070.1 NZ_CP046068:c13904-13569 [Vibrio sp. THAF191d]
MRNTKRKPVTVGEMLKLEFLEPLAISNSDLASAIGVHRNTISNLINGKTALTSDLANKLAAALGNTPEFWLNIQHAVDVW
DSKNTFEVAKKAITPLVKIESSMLAGGYALA

Nucleotide

• Length: 336 bp

>AT143507 NZ_CP061170:c904097-903837 [Microbacterium sp. HY82]
ATGAGCCGCGCGCTCGCGTTCGACAAGAACGGTTGGGAAGACTACGTCTATTGGCAAACCCAAGACCGCAAGACACTCAA
ACGGATCAACACGCTCATCGCAGACGTGCTCCGCGACCCATTCACCGGGATCGGAAAACCGGAACCACTCAAGCACATCC
TCAGCGGCGCCTGGTCGCGGCGCATCGACGAAACGAACCGACTCGTCTACTACGTCACCGACGACCACATTGTGATCCTC
CAAGCACGCGATCACTATTGA